Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
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TLDR
It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.Abstract:
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.read more
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Activated platelets can deliver mRNA regulatory Ago2•microRNA complexes to endothelial cells via microparticles
Benoit Laffont,Aurélie Corduan,Hélène Plé,Anne-Claire Duchez,Nathalie Cloutier,Eric Boilard,Patrick Provost +6 more
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Deregulation of microRNA-503 Contributes to Diabetes Mellitus–Induced Impairment of Endothelial Function and Reparative Angiogenesis After Limb Ischemia
Andrea Caporali,Marco Meloni,Christine Vollenkle,Desireee Bonci,Graciela B. Sala-Newby,Roberta Addis,Gaia Spinetti,Sergio Losa,Rachel Masson,Andy Baker,Reuven Agami,Carlos le Sage,Gainluigi Condorelli,Paolo Madeddu,Fabio Martelli,Costanza Emanueli +15 more
TL;DR: The discovery that microRNA-503 expression in ECs is upregulated in culture conditions mimicking diabetes mellitus and ischemia-associated starvation and evidence that miR-503expression is increased in ischemic limb muscles of streptozotocin-diabetic mice and inECs enriched from these muscles are suggested.
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Noncoding RNAs, cytokines, and inflammation-related diseases
José Luiz Marques-Rocha,Mirian Samblas,Fermín I. Milagro,Josefina Bressan,J. Alfredo Martínez,Amelia Marti +5 more
TL;DR: Findings in the field are summarized, highlighting those noncoding RNAs that regulate inflammation, with emphasis on recognized mediators such as TNF‐α, IL‐1,IL‐6, IL-18, intercellular adhesion molecule 1, VCAM‐1 and plasminogen activator inhibitor 1.
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Expression Profile of MicroRNAs in Young Stroke Patients
Kay Sin Tan,Arunmozhiarasi Armugam,Sugunavathi Sepramaniam,Kai Ying Lim,Karolina Dwi Setyowati,Chee Woon Wang,Kandiah Jeyaseelan +6 more
TL;DR: It is demonstrated that the peripheral blood miRNAs and their profiles can be developed as biomarkers in diagnosis and prognosis of cerebral ischaemic stroke.
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Plasma microRNAs as potential biomarkers for non-small-cell lung cancer
Jun Shen,Nevins W. Todd,Howard Zhang,Lei Yu,Xing Lingxiao,Xing Lingxiao,Yuping Mei,Maria A. Guarnera,Jipei Liao,Amy Chou,Changwan Larry Lu,Zhengran Jiang,Hong-Bin Fang,Ruth L. Katz,Feng Jiang +14 more
TL;DR: Altered expressions of the miRNAs in plasma would provide potential blood-based biomarkers for NSCLC.
References
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TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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