Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
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TLDR
It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.Abstract:
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.read more
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Journal ArticleDOI
MicroRNA-193a-3p Reduces Intestinal Inflammation in Response to Microbiota via Down-regulation of Colonic PepT1.
Xin Dai,Xi Chen,Qun Chen,Lei Shi,Hongwei Liang,Zhen Zhou,Qian Liu,Wenjing Pang,Dongxia Hou,Cheng Wang,Ke Zen,Yaozong Yuan,Chen-Yu Zhang,Lu Xia +13 more
TL;DR: It is suggested that miR-193a-3p regulation of PepT1 mediates the uptake of bacterial products and is a potent mechanism during the colonic inflammation process and may be a potent regulator of colonic PepT 1 for maintaining intestinal homeostasis.
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Serum miR-206 and other muscle-specific microRNAs as non-invasive biomarkers for Duchenne muscular dystrophy.
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MicroRNAs and cancer; an overview.
TL;DR: Modulating miRNAs, based on two major approaches (miRNA mimics and miRNA antagonists), is used for clinical development of therapeutic miRNAS and emphasizes the latest discovery in the field of miRNA research involved in cancer, biomarkers, and therapeutics.
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Serum miRNA expression profile as a prognostic biomarker of stage II/III colorectal adenocarcinoma
Jialu Li,Yang Liu,Cheng Wang,Ting Deng,Hongwei Liang,Yi-Fei Wang,Dingzhi Huang,Qian Fan,Qian Fan,Xia Wang,Tao Ning,Rui Liu,Chen-Yu Zhang,Ke Zen,Xi Chen,Yi Ba +15 more
TL;DR: Novel serum-miRNA-based biomarkers for monitoring tumor dynamics as well as for predicting disease recurrence in patients with stage II/III CRC are revealed.
Dissertation
MicroRNAs in disease and health: aberrant regulation in lung cancer and association with genomic variation
TL;DR: In this paper, the authors proposed that the human genome is encoded by human proteins but instead of encoding human proteins, the structural and binding genes are measured by non-coding binding genes.
References
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