Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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TLDR
It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.Abstract:
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.read more
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The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway
Maria Lee,Hee Jung Kim,Sang Wun Kim,Sun Ae Park,Kyung-Hee Chun,Nam Hoon Cho,Yong Sang Song,Young Tae Kim +7 more
TL;DR: Increased HOTAIR expression was associated with decreased patient survival times and may be a useful target for treatment of cervical cancer patients, according to univariate analysis.
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Atherosclerotic Conditions Promote the Packaging of Functional MicroRNA-92a-3p Into Endothelial Microvesicles
Yangyang Liu,Qian Li,Qian Li,Mohammed Rabiul Hosen,Andreas Zietzer,Anna Flender,Paula Levermann,Theresa Schmitz,Daniel Jw Frühwald,Philip Roger Goody,Georg Nickenig,Nikos Werner,Felix Jansen +12 more
TL;DR: Athrosclerotic conditions promote the packaging of endothelial miR-92a-3p into EMVs, which accelerated cell migration and proliferation in recipient ECs, which accelerate cell migration, proliferation, and blocked vascular network formation in a matrigel plug.
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Oxidative stress in prostate hyperplasia and carcinogenesis
TL;DR: Scientific evidence suggests that micronutrient supplementation may restore the antioxidant status and hence improve the clinical outcomes for patients with BPH and PCa and examines the role of OS on the molecular pathology of prostate cancer progression and treatment.
Journal ArticleDOI
Increased microRNA-146a levels in plasma of patients with newly diagnosed type 2 diabetes mellitus
Ying Rong,Wei Bao,Zhilei Shan,Jun Liu,Xuefeng Yu,Songfan Xia,Hui Gao,Xia Wang,Ping Yao,Frank B. Hu,Liegang Liu +10 more
TL;DR: Examination of circulating miRNA-146a levels in newly diagnosed type 2 diabetes mellitus patients from a Chinese Han population found that circulating miRNAs levels were significantly elevated in new-T2DM patients compared with healthy controls.
Journal ArticleDOI
Analysis of miR-205 and miR-155 expression in the blood of breast cancer patients.
Jingjing Liu,Jingjing Liu,Qixin Mao,Qixin Mao,Yan Liu,Yan Liu,Xiaomeng Hao,Xiaomeng Hao,Sheng Zhang,Sheng Zhang,Jin Zhang +10 more
TL;DR: Functional analysis showed that ectopic expression of miR-205 significantly inhibits cell proliferation and promotes apoptosis and the relationship between the expression levels of these two miRNAs and the clinicopathologic parameters of BC patients was analyzed.
References
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Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
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MicroRNA expression profiles classify human cancers
Jun Lu,Gad Getz,Eric A. Miska,Eric A. Miska,Ezequiel Alvarez-Saavedra,Justin Lamb,David Peck,Alejandro Sweet-Cordero,Alejandro Sweet-Cordero,Benjamin L. Ebert,Benjamin L. Ebert,Raymond H. Mak,Raymond H. Mak,Adolfo A. Ferrando,James R. Downing,Tyler Jacks,H. Robert Horvitz,H. Robert Horvitz,Todd R. Golub,Todd R. Golub,Todd R. Golub +20 more
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Journal Article
MicroRNA signatures in human cancers
George A. Calin,Carlo M. Croce +1 more
TL;DR: The causes of the widespread differential expression of miRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the miRNA processing machinery as discussed by the authors.
Journal Article
Oncomirs : microRNAs with a role in cancer
TL;DR: I MicroRNAs (miRNAs) are an abundant class of small non-protein-coding RNAs that function as negative gene regulators as discussed by the authors, and have been shown to repress the expression of important cancer-related genes and might prove useful in the diagnosis and treatment of cancer.
Journal ArticleDOI
Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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