Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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TLDR
It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.Abstract:
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.read more
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Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients
Edgars Endzeliņš,Andreas Berger,Vita Melne,Cristina Bajo-Santos,Kristīne Soboļevska,Artūrs Ābols,Marta Rodríguez,Daiga Santare,Anastasija Rudņickiha,Vilnis Lietuvietis,Alicia Llorente,Aija Linē +11 more
TL;DR: This study demonstrates that for some miRNA biomarkers EVs provide a more consistent source of RNA than whole plasma, while other miRNAs show better diagnostic performance when tested in the whole plasma.
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Plasma miRNAs as early biomarkers for detecting hepatocellular carcinoma
Yang Wen,Jing Han,Jianguo Chen,Jing Dong,Yongxiang Xia,Jibin Liu,Yue Jiang,Juncheng Dai,Jianhua Lu,Guangfu Jin,Jiali Han,Qingyi Wei,Hongbing Shen,Beicheng Sun,Zhibin Hu +14 more
TL;DR: A meta‐analysis revealed that four miRNAs could be used as preclinical biomarkers for HCC screening and the expression profile of the eight‐miRNA panel can be used to discriminate HCC patients from cancer‐free controls, and the four‐mi RNA panel (alone or combined with AFP) could be a blood‐based early detection biomarker for H CC screening.
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The circulating microRNA-221 level in patients with malignant melanoma as a new tumor marker
Hisashi Kanemaru,Satoshi Fukushima,Junji Yamashita,Noritoshi Honda,Rie Oyama,Asako Kakimoto,Shinichi Masuguchi,Tsuyoshi Ishihara,Yuji Inoue,Masatoshi Jinnin,Hironobu Ihn +10 more
TL;DR: Serum levels of miR-221 were significantly increased in MM patients and may be useful not only for the diagnosis of MM, but also for the differentiating MM in situ from stage I-IV MM, and for evaluating tumor progression and monitoring patients during the follow-up period.
Journal ArticleDOI
Hepatocyte-derived microRNAs as serum biomarkers of hepatic injury and rejection after liver transplantation.
Waqar R. R. Farid,Qiuwei Pan,Adriaan J. van der Meer,Petra E. de Ruiter,Vedashree Ramakrishnaiah,Jeroen de Jonge,Jaap Kwekkeboom,Harry L.A. Janssen,Herold J. Metselaar,Hugo W. Tilanus,Geert Kazemier,Luc J. W. van der Laan +11 more
TL;DR: It is shown that liver injury is associated with the release of HDmiRs into the circulation andHDmiRs are promising candidates as early, stable, and sensitive biomarkers of rejection and hepatic injury after liver transplantation.
Journal ArticleDOI
Predictive and prognostic molecular markers for cancer medicine.
Sunali Mehta,Andrew N. Shelling,Anita Muthukaruppan,Annette Lasham,Cherie Blenkiron,George Laking,Cristin G. Print +6 more
TL;DR: This review will summarize the current technologies for predicting treatment response and prognosis in cancer medicine, and outline what the future may hold, and highlight the potential importance of methods that can integrate molecular, histopathological and clinical information into a synergistic understanding of tumor progression.
References
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Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
Journal ArticleDOI
MicroRNA expression profiles classify human cancers
Jun Lu,Gad Getz,Eric A. Miska,Eric A. Miska,Ezequiel Alvarez-Saavedra,Justin Lamb,David Peck,Alejandro Sweet-Cordero,Alejandro Sweet-Cordero,Benjamin L. Ebert,Benjamin L. Ebert,Raymond H. Mak,Raymond H. Mak,Adolfo A. Ferrando,James R. Downing,Tyler Jacks,H. Robert Horvitz,H. Robert Horvitz,Todd R. Golub,Todd R. Golub,Todd R. Golub +20 more
TL;DR: A new, bead-based flow cytometric miRNA expression profiling method is used to present a systematic expression analysis of 217 mammalian miRNAs from 334 samples, including multiple human cancers, and finds the miRNA profiles are surprisingly informative, reflecting the developmental lineage and differentiation state of the tumours.
Journal Article
MicroRNA signatures in human cancers
George A. Calin,Carlo M. Croce +1 more
TL;DR: The causes of the widespread differential expression of miRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the miRNA processing machinery as discussed by the authors.
Journal Article
Oncomirs : microRNAs with a role in cancer
TL;DR: I MicroRNAs (miRNAs) are an abundant class of small non-protein-coding RNAs that function as negative gene regulators as discussed by the authors, and have been shown to repress the expression of important cancer-related genes and might prove useful in the diagnosis and treatment of cancer.
Journal ArticleDOI
Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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