Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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TLDR
It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.Abstract:
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.read more
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Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction
Jing Ai,Rong Zhang,Yue Li,Jielin Pu,Yanjie Lu,Jundong Jiao,Kang Li,Bo Yu,Zhu-Qin Li,Rongrong Wang,Li-Hong Wang,Qiang Li,Ning Wang,Hongli Shan,Zhong-Yu Li,Baofeng Yang +15 more
TL;DR: Increased circulating miR-1 level was significantly higher in plasma from AMI patients compared with non-AMI subjects and the level was dropped to normal on discharge following medication, revealing that circulating miD-1 may be a novel, independent biomarker for diagnosis of AMI.
Journal ArticleDOI
Circulating microRNAs in patients with chronic hepatitis C and non-alcoholic fatty liver disease.
Silvia Cermelli,Anna Ruggieri,Anna Ruggieri,Jorge A. Marrero,George N. Ioannou,Laura Beretta +5 more
TL;DR: Serum levels ofmiR-34a and miR-122 may represent novel, noninvasive biomarkers of diagnosis and histological disease severity in patients with chronic hepatitis C infection or non-alcoholic fatty-liver disease (NAFLD).
Journal ArticleDOI
MicroRNAs in liver disease
Gyongyi Szabo,Shashi Bala +1 more
TL;DR: Advances in the field of miRNAs in liver diseases, inflammation and cirrhosis are summarized and miRNA modulation in the liver represents a new therapeutic approach in the treatment armamentarium of hepatologists in the future.
Journal ArticleDOI
Circulating microRNAs, miR-21, miR-122, and miR-223, in patients with hepatocellular carcinoma or chronic hepatitis.
Jian Xu,Chen Wu,Xu Che,Li Wang,Dianke Yu,Tongwen Zhang,Liming Huang,Hui Li,Wen Tan,Chengfeng Wang,Dongxin Lin +10 more
TL;DR: Serum miRNAs presented in serum of patients with HCC and chronic hepatitis have strong potential to serve as novel biomarkers for liver injury but not specifically for HCC, according to Receiver‐operator characteristic (ROC) curve analyses.
Journal ArticleDOI
microRNA as a new immune-regulatory agent in breast milk
TL;DR: This study suggests the transfer of genetic material as miRNA from human to human occurs by means other than through sexual reproduction, indicating that breast milk allows dietary intake of miRNAs by infants.
References
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Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
Journal ArticleDOI
MicroRNA expression profiles classify human cancers
Jun Lu,Gad Getz,Eric A. Miska,Eric A. Miska,Ezequiel Alvarez-Saavedra,Justin Lamb,David Peck,Alejandro Sweet-Cordero,Alejandro Sweet-Cordero,Benjamin L. Ebert,Benjamin L. Ebert,Raymond H. Mak,Raymond H. Mak,Adolfo A. Ferrando,James R. Downing,Tyler Jacks,H. Robert Horvitz,H. Robert Horvitz,Todd R. Golub,Todd R. Golub,Todd R. Golub +20 more
TL;DR: A new, bead-based flow cytometric miRNA expression profiling method is used to present a systematic expression analysis of 217 mammalian miRNAs from 334 samples, including multiple human cancers, and finds the miRNA profiles are surprisingly informative, reflecting the developmental lineage and differentiation state of the tumours.
Journal Article
MicroRNA signatures in human cancers
George A. Calin,Carlo M. Croce +1 more
TL;DR: The causes of the widespread differential expression of miRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the miRNA processing machinery as discussed by the authors.
Journal Article
Oncomirs : microRNAs with a role in cancer
TL;DR: I MicroRNAs (miRNAs) are an abundant class of small non-protein-coding RNAs that function as negative gene regulators as discussed by the authors, and have been shown to repress the expression of important cancer-related genes and might prove useful in the diagnosis and treatment of cancer.
Journal ArticleDOI
Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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