Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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TLDR
It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.Abstract:
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.read more
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Identification of serum and tissue micro-RNA expression profiles in different stages of inflammatory bowel disease
Marisa Iborra,Francesca Bernuzzi,Carmen Correale,Stefania Vetrano,Gionata Fiorino,Belén Beltrán,Francesco Marabita,Massimo Locati,Antonino Spinelli,Pilar Nos,Pietro Invernizzi,Silvio Danese +11 more
TL;DR: The results suggest the existence of specific miRNA expression patterns associated with IBD and their different stages and support the utility of miRNAs as possible biomarkers and needs to be validated in a large prospective cohort.
Journal ArticleDOI
Role of microRNAs in the modulation of diabetic retinopathy
Rodolfo Mastropasqua,Lisa Toto,Francesco Cipollone,Donato Santovito,Paolo Carpineto,Leonardo Mastropasqua +5 more
TL;DR: Understanding MiRNAs implication in DR could be helpful for developing new gain- or loss- of -function strategies in order to establish effective treatments and reduce the rate of visual disability due to progression of retinopathy.
Journal ArticleDOI
MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.
C. Lisa Kurtz,Bailey C.E. Peck,Emily E. Fannin,Carine Beysen,Ji Miao,Stuart R Landstreet,Shengli Ding,Vandana Turaga,P. Kay Lund,Scott M. Turner,Sudha B. Biddinger,Kasey C. Vickers,Praveen Sethupathy +12 more
TL;DR: It is demonstrated by deep sequencing and real-time quantitative PCR that hepatic levels of Foxa2 mRNA and miR-29 are elevated in a mouse model of diet-induced insulin resistance and that miR -29 fine-tunes FOXA2-mediated activation of key lipid metabolism genes, including PPARGC1A, HMGCS2, and ABHD5.
Journal ArticleDOI
microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection.
Rimi Hamam,Arwa M. Ali,Khalid Alsaleh,Moustapha Kassem,Musaed Alfayez,Abdullah Aldahmash,Nehad M. Alajez +6 more
TL;DR: A novel approach which relies on the isolation of circulating microRNAs through an enrichment step using speed-vacuum concentration which resulted in 5-fold increase in microRNA abundance led to the identification of a novel microRNA panel which was upregulated in BC patients with potential utilization in disease diagnosis and stratification.
Journal ArticleDOI
Predictive value of circulating miR-328 and miR-134 for acute myocardial infarction
TL;DR: In this paper, the diagnostic and prognostic value of miRNAs in patients with acute myocardial infarction (AMI) was evaluated by quantitative real-time PCR in plasma samples from 359 AMI patients and 30 healthy volunteers.
References
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MicroRNA expression profiles classify human cancers
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George A. Calin,Carlo M. Croce +1 more
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Oncomirs : microRNAs with a role in cancer
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Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
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