Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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TLDR
It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.Abstract:
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.read more
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Circulating microRNAs (cmiRNAs) as novel potential biomarkers for hepatocellular carcinoma.
TL;DR: The latest findings on the role of circulating miRNAs as potential minimally invasive diagnostic and prognostic biomarkers for HCC are summarized.
Journal ArticleDOI
Vascular endothelial microparticles-incorporated microRNAs are altered in patients with diabetes mellitus.
Felix Jansen,Han Wang,David Przybilla,Bernardo S. Franklin,Andreas Dolf,P Pfeifer,Theresa Schmitz,Anna Flender,Elmar Endl,Georg Nickenig,Nikos Werner +10 more
TL;DR: Diabetes mellitus significantly alters the expression of vascular endothelial miRs in circulating endothelial MPs with potential implications on vascular heath and in vitro experiments revealed that hyperglycemia reduces the packaging of miR-126 and mi-26a into EMPs.
Journal ArticleDOI
MicroRNAs in retinoblastoma: Potential diagnostic and therapeutic biomarkers.
Khodayar Golabchi,Rahim Soleimani-Jelodar,Nazila Aghadoost,Fatemeh Momeni,Abdullah Moridikia,Javid Sadri Nahand,Aria Masoudifar,Hasan Razmjoo,Hamed Mirzaei +8 more
TL;DR: A variety of tissue‐specific and circulating miRNAs involved in R b pathogenesis which could be used as diagnostic, prognostic, and therapeutic biomarkers in Rb patients are summarized.
Journal ArticleDOI
Circulating miR-30a, miR-195 and let-7b Associated with Acute Myocardial Infarction
Guangwen Long,Feng Wang,Quanlu Duan,Shenglan Yang,Fuqiong Chen,Wei Gong,Xu Yang,Yan Wang,Chen Chen,Dao Wen Wang +9 more
TL;DR: The results imply that the plasma concentration ofmiR-30a, miR-195 and let-7b can be potential indicators for AMI.
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Plasma miRNAs as biomarkers to identify patients with castration-resistant metastatic prostate cancer.
Akira Watahiki,Robyn Jane Macfarlane,Martin E. Gleave,Francesco Crea,Yuzhuo Wang,Cheryl D. Helgason,Kim N. Chi +6 more
TL;DR: The results demonstrate that plasma levels of selected miRNAs are potential biomarkers to differentiate localized PCa and mCRPC.
References
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MicroRNA expression profiles classify human cancers
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Journal Article
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George A. Calin,Carlo M. Croce +1 more
TL;DR: The causes of the widespread differential expression of miRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the miRNA processing machinery as discussed by the authors.
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Oncomirs : microRNAs with a role in cancer
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Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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