Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
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Book ChapterDOI
Deuterated peptides and proteins: structure and dynamics studies by MAS solid-state NMR.
Bernd Reif,Bernd Reif,Bernd Reif +2 more
TL;DR: Perdeuteration and back substitution of exchangeable protons in microcrystalline proteins, in combination with recrystallization from D(2)O-containing buffers, significantly reduce ( 1)H, (1)H dipolar interactions.
Journal ArticleDOI
Resolving the two monolayers of a lipid bilayer in giant unilamellar vesicles using deuterium nuclear magnetic resonance.
TL;DR: It was likewise possible to resolve two quadrupole splittings when Staphylococcus aureus delta-toxin, a surface-active peptide known to influence lipid head-group orientational ordering, was added to the exterior vesicular solution only, indicating that delta-Toxin upon binding remains confined to one monolayer of the lipid bilayer and does not traverse the membrane.
Journal ArticleDOI
Membrane lipid phase behaviour and lipid-protein interactions.
TL;DR: The lipid constituents of all biological membranes are arranged in a bilayer configuration in which the polar groups are located on the outside, in contact with the aqueous medium, and the hydrocarbon substituents are oriented toward the interior to form a hydrophobic domain that excludes water.
Journal ArticleDOI
Memory effects across surfactant mesophases
TL;DR: Surprising memory effects are found across mesophase transitions that provide clues to the structure of the various phases in the aqueous non-ionic surfactant system C(12)E(6)/water.
Journal ArticleDOI
The specificity of monoglyceride–protein interactions and mechanism of the protein induced L β to coagel phase transition
TL;DR: The results show that lysozyme inserts efficiently into all monolayers tested, including pure monoglyceride layers, and a large exothermic binding enthalpy was observed which was found to depend on the nature of the monoglycersides but not of the proteins.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.