Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
Citations
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Saponin-cholesterol interaction in the multibilayers of egg yolk lecithin as studied by deuterium nuclear magnetic resonance: digitonin and its analogues.
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Structure of Escherichia coli membranes. Phospholipid conformation in model membranes and cells as studied by deuterium magnetic resonance.
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Applications of NMR to membrane proteins.
TL;DR: Some of the recent developments that advance the application of NMR to membrane proteins, with emphasis on structural studies in detergent-free, lipid bilayer samples that resemble the native environment are reviewed.
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The fidelity of response by nitroxide spin probes to changes in membrane organization: the condensing effect of cholesterol.
Michael G. Taylor,Ian C.P. Smith +1 more
TL;DR: The response of doxyl fatty acid spin probes in egg lecithin bilayers to added cholesterol is compared with results from 2 H-NMR, and a direct measure of the perturbing effect of a spin label is made by comparing the deuterium quadrupole splittings in eggLeCithin-cholesterol bilayers for stearic acid with and without an attached doXyl moiety.
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Depth-Dependent Solvent Relaxation in Membranes: Wavelength-Selective Fluorescence as a Membrane Dipstick
TL;DR: Evidence is provided of depth-dependent solvent relaxation which can be used as a membrane dipstick and of chemically identical fluorophores, varying solely in terms of their localization at different depths in the membrane, experience very different local environments, as judged by wavelength-selective fluorescence parameters.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.