Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
Citations
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A calorimetry and deuterium NMR study of mixed model membranes of 1-palmitoyl-2-oleylphosphatidylcholine and saturated phosphatidylcholines
TL;DR: 2H-NMR spectroscopy has been used to further study mixed model membranes composed of POPC and DPPC, in which either lipid has been labeled with deuterium in the 2-, 10- or 16-position of the palmitoyl chain(s) or in the N-methyls of the choline head group.
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Stabilization of HII phases by low levels of diglycerides and alkanes: an NMR, calorimetric, and x-ray diffraction study
TL;DR: In this article, X-ray diffraction data showed that DPG decreases the lattice constant of the H{sub II} phase at constant temperature, which is consistent with localization of perdeuterated alkane molecules to the periphery of H{ sub II} tubes and to the hydrophobic interstices between the tubes.
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Solid-state NMR approaches for studying the interaction of peptides and proteins with membranes.
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Evidence for protein-associated lipids from deuterium nuclear magnetic resonance studies of rhodopsin-dimyristoylphosphatidylcholine recombinants.
TL;DR: Extraction of the oriented 2H NMR spectrum from its powder spectrum showed that the presence of proteins does not modify the distribution of quadrupole splittings but does produce both homogeneous and inhomogeneous broadening.
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Cholesterol Dynamics in Membranes of Raft Composition: A Molecular Point of View from 2H and 31P Solid-State NMR
TL;DR: Cholesterol order parameters greater than 0.8 are observed, indicating that the sterol is in a very motionally restricted environment in the temperature range 0-60 degrees C, and strongly suggests that cholesterol could be in fast exchange, at the NMR time scale (milli- to microseconds), between two or more membrane regions of different dynamics.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.