Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
Citations
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Structural Analysis of the Protein/Lipid Complexes Associated with Pore Formation by the Bacterial Toxin Pneumolysin
Boyan B. Bonev,Robert J.C. Gilbert,Robert J.C. Gilbert,Peter W. Andrew,Olwyn Byron,Anthony Watts +5 more
TL;DR: The effect of pneumolysin on lipid membranes is a complex combination of pore formation within the bilayer, extraction of lipid into free oligomeric complexes, aggregation and fusion of liposomes, and the destabilization of membranes leading to formation of small vesicles.
Journal ArticleDOI
Lipid bilayer thickness determines cholesterol's location in model membranes
Drew Marquardt,Drew Marquardt,Frederick A. Heberle,Frederick A. Heberle,Denise V. Greathouse,Roger E. Koeppe,Robert F. Standaert,Robert F. Standaert,Brad Van Oosten,Thad A. Harroun,Jacob J. Kinnun,Justin A. Williams,Stephen R. Wassall,John Katsaras +13 more
TL;DR: Using a combination of neutron scattering and solid-state 2H NMR, the authors determined the location and orientation of cholesterol in PC model membranes having fatty acids of different lengths and degrees of unsaturation.
Journal ArticleDOI
First observation of amino acid side chain dynamics in membrane proteins using high field deuterium nuclear magnetic resonance spectroscopy.
TL;DR: The results strongly suggest that it should now be possible to observe in great detail the motions of any type of amino acid side chain in membrane proteins, including the effects of lipid composition on protein dynamics.
Journal ArticleDOI
Acyl chain conformational ordering in liquid-crystalline bilayers: comparative FT-IR and 2H-NMR studies of phospholipids differing in headgroup structure and chain length
TL;DR: In this article, the integral band intensities were converted to the number of gauche conformers per acyl chain using the calibration factors published by Senak et al. (1991).
Journal ArticleDOI
Deuterium Relaxation and Molecular Dynamics in Lipid Bilayers
TL;DR: In this paper, the quadrupolar relaxation of deuterium-labeled lipid bilayers has been analyzed using standard Redfield theory and discussed with regard to the problem of chain segmental motion and order in membranes.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.