Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
Citations
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Calorimetric studies on phospholipid―water systems. II: Study of water behavior
C Grabielle-Madelmont,R Perron +1 more
Journal ArticleDOI
Effects of temperature variation and phenethyl alcohol addition on acyl chain order and lipid organization in Escherichia coli derived membrane systems. A 2H- and 31P-NMR study.
TL;DR: It is concluded that while both increasing temperature and addition of phenethyl alcohol can affect membrane integrity, in the former case this is due to the induction of non-bilayer lipid structures, whereas in the latter cases this is caused by an increase in membrane fluidity.
Journal ArticleDOI
A 2H-NMR analysis of dihydrosterculoyl-containing lipids in model membranes: structural effects of a cyclopropane ring.
TL;DR: This value represents a discontinuity in the positional dependence of the molecular order parameter for the sn-2 chain of PDSPC, indicating that the cyclopropane ring provides a rigid barrier separating the lipid bilayer into two regions: an ordered region from the bilayer surface to the site of the cycling ring and a much more disordered region thereafter to the center of the bilayers.
Journal ArticleDOI
Deuterium solid-state nuclear magnetic resonance studies of methyl group dynamics in bacteriorhodopsin and retinal model compounds: evidence for a 6-s-trans chromophore in the protein.
Valérie Copié,Ann E. McDermott,Kebede Beshah,John C. Williams,Marijke Spijker-Assink,R. Gebhard,Johan Lugtenburg,Judith Herzfeld,Robert G. Griffin +8 more
TL;DR: Results suggest that methyl rotor motions can be used to probe the van der Waals contact between a ligand and a protein binding pocket in a membrane protein bacteriorhodopsin regenerated with CD3-labeled retinal.
Journal ArticleDOI
Interactions of cholesterol with the membrane lipid matrix. A solid state NMR approach.
A. Léonard,Erick J. Dufourc +1 more
TL;DR: The use of deuterium labels both on the cholesterol fused ring system and on the lipid chains in conjunction with solid stateDeuterium nuclear magnetic resonance (2H-NMR) afforded to monitor the degree of ordering of both molecules in a mixed system.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.