Deuterium magnetic resonance: theory and application to lipid membranes.
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TLDR
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.Abstract:
Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerstrom & Lindblom, 1977).read more
Citations
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Lanosterol and cholesterol have different effects on phospholipid acyl chain ordering
TL;DR: The 14- methyl group prevents lanosterol from causing the increase in motional order of the phospholipid hydrocarbon chains characteristic of cholesterol, and suggests little disorganization of the membrane structure due to the 14-methyl group.
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Pressure effects on the structure and phase behavior of DMPC-gramicidin lipid bilayers: a synchrotron SAXS and 2H-NMR spectroscopy study.
J. Eisenblätter,R. Winter +1 more
TL;DR: The data show that, depending on the GD concentration, the structure of the temperature- and pressure-dependent lipid phases is significantly altered by the insertion of the polypeptide, and a p,T-phase diagram could be obtained for intermediate GD concentrations.
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Photoreceptor rhodopsin: structural and conformational study of its chromophore 11-cis retinal in oriented membranes by deuterium solid state NMR.
G Grobner,G Choi,Ian J. Burnett,Clemens Glaubitz,P. J. E. Verdegem,Johan Lugtenburg,Anthony Watts +6 more
TL;DR: An ab initio solid state 2H NMR approach was used to determine the orientation and conformation of retinal within the binding pocket of this membrane bound receptor, and produced an overall picture for the three dimensional structure of the polyene chain of the chromophore in the protein binding pocket around the Schiff base attachment site.
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Interaction of the neuronal marker dye FM1-43 with lipid membranes: Thermodynamics and lipid ordering
Uwe Schote,Joachim Seelig +1 more
TL;DR: In this paper, the authors used high sensitivity isothermal titration calorimetry (ITC) and deuterium magnetic resonance (2H-NMR) to determine the adsorption/partition equilibrium of FM1-43.
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Mixtures of a series of homologous hydrophobic peptides with lipid bilayers: a simple model system for examining the protein-lipid interface.
TL;DR: 1,2-Dimyristoyl-sn-glycero-3-phosphocholine bilayers and tripeptides of the form Ala-X-Ala-O-tert-butyl are used as a model system to probe the influence of amino acid side-chain substitution on the insertion of peptides into membranes and the behavior of peptide/bilayer mixtures.
References
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Book
Principles of magnetic resonance
TL;DR: In this article, the effect of changing the precession frequency of the magnetic field has been studied using NMR to study rate properties. But the effect is limited to the case of double and double resonance.
Journal ArticleDOI
Molecular motion in spin-labeled phospholipids and membranes
Book
Spin Labeling: Theory And Applications
TL;DR: This special topics volume represents a complete update on new theoretical aspects and applications of the spin-label method and includes an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations.