Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
Citations
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Journal ArticleDOI
Embryonic stem cell biology.
Michael K. Fritsch,Don B. Singer +1 more
TL;DR: The ability to generate relatively pure populations of specifically differentiated cells for transplantation has markedly improved and it is hoped that soon researchers will overcome the biologic impediments to successful treatment of human disease with ES cell-derived cells.
Journal ArticleDOI
A perspective on stem cell modeling of amyotrophic lateral sclerosis
A Sophie de Boer,Kevin Eggan +1 more
TL;DR: Recent advances in the development of stem cell models for the study of ALS are reviewed and the progress toward therapeutic development derived from these stem cell based assays is discussed.
Posted ContentDOI
Rostrocaudal Patterning and Neural Crest Differentiation of Human Pre-Neural Spinal Cord Progenitors in vitro
Fay Cooper,George E. Gentsch,Richard Mitter,Camille Bouissou,Lyn Healy,Ana Hernandez-Rodriguez,James C. Smith,Andreia S Bernardo,Andreia S Bernardo +8 more
TL;DR: The generation of NMP-derived pre-neural progenitors (PNPs) with stem cell-like self-renewal capacity and a clock that can be halted at the thoracic level for up to 30 passages by blocking the trunk-to-tail transition through GDF11-mediated signal inhibition.
Journal ArticleDOI
Synthetic small molecules that induce neuronal differentiation in neuroblastoma and fibroblast cells
TL;DR: Observations made in the present study suggest that Nz and Nzl will serve as chemical tools to generate specific populations of neuronal cells from readily available and simply manageable cells.
Journal ArticleDOI
ECEL1 mutation implicates impaired axonal arborization of motor nerves in the pathogenesis of distal arthrogryposis.
Kenichi Nagata,Sumiko Kiryu-Seo,Hiromi Tamada,Fumi Okuyama-Uchimura,Hiroshi Kiyama,Takaomi C. Saido +5 more
TL;DR: Analyzing developmental motor innervation and neuromuscular junction formation in limbs of the rodent homologue damage-induced neuronal endopeptidase (DINE)-deficient mouse provided evidence that DINE gene disruption leads to insufficient arborization of motor nerves after arriving at the skeletal muscle.
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
Journal ArticleDOI
Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.