Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Ptch2 mediates the Shh response in Ptch1 −/− cells
TL;DR: The finding that this response can be inhibited by the Shh-blocking antibody 5E1 indicates that the ShH response in Ptch1–/– cells remains ligand dependent, and it is found that Shh induces a strong response in ptch1-/–;Shh–/- cells, and that Ptch2 mediates the response to Shh in the absence of Ptch 1.
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Characterization of last-order premotor interneurons by transneuronal tracing with rabies virus in the neonatal mouse spinal cord.
TL;DR: Transneuronal tracing with RV, combined with an immunohistochemical detection of neuronal determinants, allows a very specific mapping of motor networks involved in the control of single muscles.
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Frizzled3 controls axonal development in distinct populations of cranial and spinal motor neurons
TL;DR: In vivo evidence is provided that developing neurons with long-range axons are programmed to die unless their axons arrive at intermediate targets on schedule, and planar cell polarity (PCP) signaling in motor axon growth is implicate.
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Inhibition of Sirt1 promotes neural progenitors toward motoneuron differentiation from human embryonic stem cells
TL;DR: This study provides an alternative method for the production of transplantable motoneurons, a key requirement in the development of hESC-based cell therapy in motoneuron disease.
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Generation of spinal motor neurons from human fetal brain-derived neural stem cells: role of basic fibroblast growth factor.
TL;DR: It is reported that in‐vitro‐expanded human fetal forebrain‐derived NSCs can generate cholinergic neurons with spinal motor neuron properties when treated with bFGF within a specific time window, indicating that bF GF can play an important role in modulating plasticity and neuronal fate of human N SCs.
References
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Journal ArticleDOI
Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.