Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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TLDR
It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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In vitro recapitulation of neural development using embryonic stem cells: from neurogenesis to histogenesis.
Keiko Muguruma,Yoshiki Sasai +1 more
TL;DR: The ability of ES cells to generate not only specific neuronal populations but also functional neural tissues by recapitulating microenvironments in early mammalian development is reviewed.
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Chemical approaches to studying stem cell biology.
TL;DR: Chemical approaches have recently provided a number of small molecules that can be used to control cell self-renewal, lineage differentiation, reprogramming and regeneration, and this strategy is promising to be a new frontier for drug development aimed at endogenous stem cell modulation.
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Hedgehog Pathway Agonism: Therapeutic Potential and Small‐Molecule Development
TL;DR: The goal of this review is to highlight the recent evidence supporting the therapeutic potential of Hh pathway activators and to provide a comprehensive overview of small‐molecule pathway agonists.
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Self-renewal and cell lineage differentiation strategies in human embryonic stem cells and induced pluripotent stem cells.
TL;DR: This review highlights the current strategies used to maintain stem cell pluripotency (a measure of stem cell quality), as well as providing an overview of the various differentiation strategies being used to generate cells from all three germ lineages.
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Stem cell therapy for retinal degeneration: retinal neurons from heterologous sources.
Ani M. Das,Xing Zhao,Iqbal Ahmad +2 more
TL;DR: The current state of knowledge about obtaining retinal cells from heterologous sources, including ES cells is reviewed.
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.