Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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V2a interneuron differentiation from mouse and human pluripotent stem cells
Jessica C. Butts,Jessica C. Butts,Nisha Iyer,Nick White,Russell E. Thompson,Shelly E. Sakiyama-Elbert,Todd C. McDevitt,Todd C. McDevitt +7 more
TL;DR: How to produce V2a interneurons from mouse and human pluripotent stem cells (PSCs), as well as strategies to characterize and mature the cells for further analysis are described, which could lead to novel regenerative therapies for traumatic injuries and drug targets for chronic degenerative diseases.
Journal ArticleDOI
Polycomb Repressive Complex 2 Regulates Lineage Fidelity during Embryonic Stem Cell Differentiation
TL;DR: A critical role for PRC2 is demonstrated in safeguarding lineage decisions and in protecting genes against inappropriate DNA methylation in both ESCs and in SMNs.
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Stage-dependent Olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells.
Soojung Shin,Haipeng Xue,Haipeng Xue,Mark P. Mattson,Mahendra S. Rao,Mahendra S. Rao,Mahendra S. Rao +6 more
TL;DR: It is shown that ES cell-derived Olig2 + cells can give rise to both motor neurons and oligodendrocytes, depending on the time at which differentiation is initiated, and that human ES cells to Sonic hedgehog likewise resulted in enhanced motor neuron differentiation.
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Mouse embryonic stem cell-derived cells reveal niches that support neuronal differentiation in the adult rat brain.
Guadalupe Maya-Espinosa,Omar Collazo-Navarrete,Diana Millán-Aldaco,Marcela Palomero-Rivero,Gilda Guerrero-Flores,René Drucker-Colín,Luis Covarrubias,Magdalena Guerra-Crespo +7 more
TL;DR: It is determined that the adult substantia nigra pars compacta, considered a non‐neurogenic area, harbors a robust neurogenic environment, therefore, neurally uncommitted cells derived from ESCs can detect regions that support neuronal differentiation within the adult brain, a fundamental step for the development of stem cell‐based replacement therapies.
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Derivation of Endodermal Progenitors From Pluripotent Stem Cells.
TL;DR: This review critically review the emerging literature aiming to achieve the efficient and reproducible in vitro derivation of endodermal progenitors such as pancreas, liver and lung precursor cells in terms of key transcription factors and signaling pathways.
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.