Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Progress and promise: the current status of spinal muscular atrophy therapeutics.
TL;DR: The identification of therapeutic targets and the development of suitable animal models for preclinical testing have resulted in increased drug development efforts in the past ten years, and the current status of many programs, including those designed to activate SMN2 gene expression, modulate splicing ofSMN2 preRNAs, stabilize SMN protein, replace SMN1, provide neuroprotective support, and transplant neural cells are reviewed.
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Human Motor Neurons Generated from Neural Stem Cells Delay Clinical Onset and Prolong Life in ALS Mouse Model
TL;DR: It is shown that intrathecal transplantation of human motor neurons derived from neural stem cells (NSCs) in spinal cord of the SOD1G93A mouse ALS model delayed disease onset and extended life span of the animals, and this treatment modality might be of value in the treatment of ALS patients without significant adverse effects.
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MicroRNA filters Hox temporal transcription noise to confer boundary formation in the spinal cord.
Chung-Jung Li,Tian Hong,Ying-Tsen Tung,Ya-Ping Yen,Ya-Ping Yen,Ho-Chiang Hsu,Ya-Lin Lu,Mien Chang,Qing Nie,Jun-An Chen,Jun-An Chen +10 more
TL;DR: This work uncovers two feed-forward Hox-miRNA loops accounting for the precocious and noisy Hoxa5 expression, and identifies mir-27 as a major regulator coordinating the temporal delay and spatial boundary of Hox protein expression.
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Novel α-tubulin mutation disrupts neural development and tubulin proteostasis
M. Gartz Hanson,Jayne Aiken,Daniel V. Sietsema,David Sept,Emily A. Bates,Lee Niswander,Jeffrey K. Moore +6 more
TL;DR: Using a forward genetic screen in mice, a missense mutation in Tuba1a α-tubulin is identified that disrupts cortical and motor neuron development and is found to be depleted from the cell lysate and from microtubules, thereby altering ratios of α- Tubulin isotypes.
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Apoptosis of limb innervating motor neurons and erosion of motor pool identity upon lineage specific dicer inactivation.
Jun-An Chen,Hynek Wichterle +1 more
TL;DR: The results indicate that miRNAs are an integral part of the genetic program controlling motor neuron survival and acquisition of subtype specific properties.
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.