Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Strategies for delineating spinal locomotor rhythm-generating networks and the possible role of Hb9 interneurones in rhythmogenesis
TL;DR: It is suggested that Hb9 interneurones could be involved in an asymmetric model of locomotor rhythmogenesis through projections of electrotonically coupled rhythm-generating modules to flexor pattern formation half-centres.
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Saltatory remodeling of Hox chromatin in response to rostrocaudal patterning signals
Esteban O. Mazzoni,Shaun Mahony,Shaun Mahony,Mirza Peljto,Tulsi Patel,Seraphim R. Thornton,Scott McCuine,Christopher Reeder,Laurie A. Boyer,Richard A. Young,David K. Gifford,Hynek Wichterle +11 more
TL;DR: In this article, the authors demonstrate that Hox chromatin is subdivided into discrete domains that are controlled by rostrocaudal patterning signals that trigger rapid, domain-wide clearance of repressive histone H3 Lys27 trimethylation (H3K27me3) polycomb modifications.
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How to make spinal motor neurons
Brandi N. Davis-Dusenbery,Brandi N. Davis-Dusenbery,Luis A. Williams,Joseph R. Klim,Joseph R. Klim,Kevin Eggan,Kevin Eggan,Kevin Eggan +7 more
TL;DR: This Primer discusses how the logic of spinal motor neuron development has been applied to allow generation of motor neurons either from pluripotent stem cells by directed differentiation and transcriptional programming, or from somatic cells by direct lineage conversion.
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Differentiation of Insulin-Producing Cells from Human Neural Progenitor Cells
TL;DR: It is shown that brain-derived human neural progenitor cells, exposed to a series of signals that regulate in vivo pancreatic islet development, form clusters of glucose-responsive insulin-producing cells (IPCs), and production of IPCs solely through extracellular factor modulation in the absence of genetic manipulations may promote strategies to derive transplantable islet-replacement tissues.
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Functional diversity of ESC-derived motor neuron subtypes revealed through intraspinal transplantation.
Mirza Peljto,Jeremy S. Dasen,Esteban O. Mazzoni,Thomas Jessell,Thomas Jessell,Hynek Wichterle +5 more
TL;DR: It is shown that mouse ESCs can be directed to form highly specific motor neuron subtypes in the absence of added factors, through a differentiation program that relies on endogenous Wnts, FGFs, and Hh-mimicking the normal program of motor neurons subtype differentiation.
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.