Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Journal ArticleDOI
An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming
Mathieu Gabut,Payman Samavarchi-Tehrani,Payman Samavarchi-Tehrani,Xinchen Wang,Valentina Slobodeniuc,Dave O'Hanlon,Hoon Ki Sung,Manuel Alvarez,Shaheynoor Talukder,Qun Pan,Esteban O. Mazzoni,Stéphane Nedelec,Hynek Wichterle,Knut Woltjen,Timothy P. Hughes,Peter W. Zandstra,Andras Nagy,Andras Nagy,Jeffrey L. Wrana,Jeffrey L. Wrana,Benjamin J. Blencowe +20 more
TL;DR: It is shown that the ESC-specific isoform of FOXP1 stimulates the expression of transcription factor genes required for pluripotency, including OCT4, NANOG, NR5A2, and GDF3, while concomitantly repressing genes necessary for ESC differentiation.
Journal ArticleDOI
FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons
Lorenzo Errichelli,Lorenzo Errichelli,Stefano Dini Modigliani,Pietro Laneve,Alessio Colantoni,Ivano Legnini,Davide Capauto,Davide Capauto,Alessandro Rosa,Alessandro Rosa,Riccardo De Santis,Riccardo De Santis,Rebecca Scarfò,Giovanna Peruzzi,Lei Lu,Elisa Caffarelli,Elisa Caffarelli,Neil A. Shneider,Mariangela Morlando,Irene Bozzoni +19 more
TL;DR: It is demonstrated that FUS regulates circRNA biogenesis by binding the introns flanking the back-splicing junctions and that this control can be reproduced with artificial constructs.
Journal ArticleDOI
Next-Generation Regenerative Medicine: Organogenesis from Stem Cells in 3D Culture
TL;DR: Recent discoveries about the dynamic patterning and structural self-formation of complex organ buds in 3D stem cell culture, including the generation of various neuroectodermal and endodermal tissues are covered.
Book ChapterDOI
Defined conditions for neural commitment and differentiation.
Qi-Long Ying,Austin Smith +1 more
TL;DR: This simple culture system is amenable to cellular and molecular dissection, promising to yield new insights into the mechanism underlying neural determination in mammals and perhaps to deliver the goal of "directed" homogeneous differentiation of ES cells.
Journal ArticleDOI
Retinoic-acid-concentration-dependent acquisition of neural cell identity during in vitro differentiation of mouse embryonic stem cells
TL;DR: It is suggested that the concentration of RA strictly and simultaneously regulates the neuralization and positional specification during differentiation of mouse ES cells and that it may be possible to use it to establish a strategy for controlling the identity of ES-cell-derived neural cells.
References
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TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.