Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Assessment of the stromal contribution to Sonic Hedgehog-dependent pancreatic adenocarcinoma
Helene Damhofer,Jan Paul Medema,Veronique L. Veenstra,Liviu Badea,Irinel Popescu,Henk Roelink,Maarten F. Bijlsma,Maarten F. Bijlsma +7 more
TL;DR: Insight is provided into the role of the activated stroma in PDAC, and how SHH acts to mediate this response, and several candidates that are interesting therapeutic targets for a disease for which treatment options are still inadequate are yielded.
Journal ArticleDOI
Plxdc2 is a mitogen for neural progenitors
TL;DR: In the chick neural tube, misexpression of Plxdc2 increases proliferation and alters patterns of neurogenesis, resulting in neural tube thickening at early stages, demonstrating a cell non-autonomous function.
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Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
TL;DR: This two-step differentiation protocol provides an efficient way to differentiate mES cells into spinal motor neurons and shows that GFP+ cells express the motor neuron specific markers, Islet-1 and choline acetyltransferase (ChAT).
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In vivo mechanical condition plays an important role for appearance of cartilage tissue in ES cell transplanted joint.
TL;DR: It is suggested that the environment plays an important role for ES cells in the process of repairing cartilage tissue in vivo.
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Developmental and functional nature of human iPSC derived motoneurons.
Marianne Stockmann,Leonhard Linta,Karl J. Föhr,Anja Boeckers,Albert C. Ludolph,Georges F. Kuh,Patrick T Udvardi,Christian Proepper,Alexander Storch,Alexander Storch,Alexander Kleger,Stefan Liebau,Tobias M. Boeckers +12 more
TL;DR: Since the first discovery and invention of the induced pluripotent stem cell (iPSC) technology, it is now possible to analyze and study cell development and differentiation on the basis of a gene defect in patient specific settings.
References
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Journal ArticleDOI
Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.