Directed Differentiation of Embryonic Stem Cells into Motor Neurons
Reads0
Chats0
TLDR
It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
Citations
More filters
Journal ArticleDOI
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Kiichi Watanabe,Morio Ueno,Daisuke Kamiya,Ayaka Nishiyama,Michiru Matsumura,Takafumi Wataya,Jun Takahashi,Satomi Nishikawa,Shin-Ichi Nishikawa,Keiko Muguruma,Yoshiki Sasai +10 more
TL;DR: Application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency and facilitates subcloning after gene transfer, and enables SFEB-cultured hES Cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors.
Journal ArticleDOI
Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons.
John T. Dimos,Kit T. Rodolfa,Kathy K. Niakan,Laurin M. Weisenthal,Hiroshi Mitsumoto,Wendy K. Chung,Wendy K. Chung,Gist F. Croft,Genevieve Saphier,Rudy Leibel,Robin Goland,Hynek Wichterle,Christopher E. Henderson,Kevin Eggan +13 more
TL;DR: Induced pluripotent stem cells are generated from an 82-year-old woman diagnosed with a familial form of amyotrophic lateral sclerosis and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS.
Journal ArticleDOI
Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development.
Charles E. Murry,Gordon Keller +1 more
TL;DR: The potential to generate virtually any differentiated cell type from embryonic stem cells (ESCs) offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine, but it is essential to be able to control ESC differentiation and to direct the development of these cells along specific pathways.
Journal ArticleDOI
Spinal-cord injury
TL;DR: An overview of the newer therapeutic interventions employed in the care of the spinal cord injured individual and the theoretical rationale supporting them is presented.
Journal ArticleDOI
Induced pluripotent stem cells from a spinal muscular atrophy patient
Allison D. Ebert,Junying Yu,Ferrill F. Rose,Virginia B. Mattis,Christian L. Lorson,James A. Thomson,Clive N. Svendsen +6 more
TL;DR: This is the first study to show that human induced pluripotent stem cells can be used to model the specific pathology seen in a genetically inherited disease and represents a promising resource to study disease mechanisms, screen new drug compounds and develop new therapies.
References
More filters
Journal ArticleDOI
Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons.
TL;DR: Molecular characterization of neuron subtypes generated from in vitro differentiation of embryonic stem (ES) cells should considerably facilitate the identification of new genes expressed by restricted neuronal cell lineages.
Journal Article
Survival in vitro of motoneurons identified or purified by novel antibody-based methods is selectively enhanced by muscle-derived factors.
TL;DR: Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody as discussed by the authors, which constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos and survived for at least 5 days in the presence of muscle extract, but not in its absence.
Journal ArticleDOI
OTX2 homeoprotein in the developing central nervous system and migratory cells of the olfactory area
TL;DR: The distribution of OTX2 during mouse development is analyzed, finding the protein is essentially confined to anterior neuroectoderm corresponding to presumptive fore- and midbrain and subsequent distribution in forebrain, midbrain, developing isthmo-cerebellum and posterior central nervous system is analyzed in detail.
Journal ArticleDOI
Noggin and chordin have distinct activities in promoting lineage commitment of mouse embryonic stem (ES) cells.
Theresa E. Gratsch,K. Sue O'Shea +1 more
TL;DR: Data suggest that, consistent with their different expression fields, noggin and chordin may play distinct roles in patterning the early mouse embryo.
Book ChapterDOI
Retinoids and related signals in early development of the vertebrate central nervous system.
TL;DR: Hox gene-mediated modulation of the dorsoventral program of growth and neurogenesis provides an elegant mechanism, whereby retinoids and other signals could affect or generate positional information.