Directed Differentiation of Embryonic Stem Cells into Motor Neurons
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It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.About:
This article is published in Cell.The article was published on 2002-08-09 and is currently open access. It has received 1763 citations till now. The article focuses on the topics: Motor neuron & Neurogenesis.read more
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Cellular activity of Wharton's Jelly‐derived mesenchymal stem cells on electrospun fibrous and solvent‐cast film scaffolds
TL;DR: Collagen-coated electrospun PCL have potential for being used in neural tissue engineering because of its bioactive and three-dimensional structure which enhance viability and differentiation of WJMSCs.
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Development of MAP4 Kinase Inhibitors as Motor Neuron-Protecting Agents.
Pieter H. Bos,Emily Rhodes Lowry,Jonathon Costa,Sebastian Thams,Alejandro Garcia-Diaz,Arie Zask,Hynek Wichterle,Brent R. Stockwell +7 more
TL;DR: Prostetin/12k emerged as an exceptionally potent, metabolically stable, and blood-brain barrier-penetrant compound that is well suited for future testing in animal models of neurodegeneration.
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Directed Differentiation of V3 Interneurons from Mouse Embryonic Stem Cells.
Hao Xu,Shelly E. Sakiyama-Elbert +1 more
TL;DR: A novel induction protocol for V3 INs from mouse embryonic stem cells is developed and reveals the importance of Shh signaling duration in the dorsal-ventral patterning of the neural tube, and it provides a method to obtain V3 Ins for future studies to allow better understanding their role in rewiring and regeneration after spinal cord injury.
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Subtype Diversification and Synaptic Specificity of Stem Cell-Derived Spinal Interneurons.
Phuong T. Hoang,Joshua I. Chalif,Jay B. Bikoff,Thomas M. Jessell,George Z. Mentis,Hynek Wichterle +5 more
TL;DR: An embryonic stem cell-based system to model subtype diversification of V1 interneurons, a class of spinal neurons comprising four clades collectively containing dozens of molecularly distinct neuronal subtypes, and shows that Renshaw cells are intrinsically programmed to migrate to species-specific laminae upon transplantation and to form subtype-specific synapses with motor neurons.
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How big is the myelinating orchestra? Cellular diversity within the oligodendrocyte lineage: facts and hypotheses
TL;DR: This journey will review and discuss current knowledge about OL development and function in the brain and spinal cord, and address some specific questions: do multiple OL subtypes exist in the CNS?
References
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Mammalian neural stem cells.
TL;DR: Before the full potential of neural stem cells can be realized, the authors need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells.
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Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function.
Chin Chiang,Ying Litingtung,Eric Lee,Keith E. Young,Jeffrey L Corden,Heiner Westphal,Philip A. Beachy +6 more
TL;DR: Targeted gene disruption in the mouse shows that the Sonic hedgehog(Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs.
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Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TL;DR: It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.
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Neuronal specification in the spinal cord: inductive signals and transcriptional codes
TL;DR: The mechanisms that specify the identity of neural cells have been examined in many regions of the nervous system and reveal a high degree of conservation in the specification of cell fate by key signalling molecules.
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In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.