Generation of functional antibodies for mammalian membrane protein crystallography
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TLDR
These protocols are presented to generate functional mouse antibodies for the membrane protein crystallography, which have enabled us to solve crystal structures of mammalian receptors and transporters complexed with antibody fragments.About:
This article is published in Current Opinion in Structural Biology.The article was published on 2013-08-01 and is currently open access. It has received 41 citations till now. The article focuses on the topics: Integral membrane protein & Membrane protein.read more
Citations
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Journal ArticleDOI
Crystal structure of the anion exchanger domain of human erythrocyte band 3
Takatoshi Arakawa,Takami Kobayashi-Yurugi,Yilmaz Alguel,Hiroko Iwanari,Hinako Hatae,Momi Iwata,Yoshito Abe,Tomoya Hino,Chiyo Ikeda-Suno,Hiroyuki Kuma,Dongchon Kang,Takeshi Murata,Takao Hamakubo,Alexander D. Cameron,Takuya Kobayashi,Naotaka Hamasaki,So Iwata +16 more
TL;DR: The crystal structure of the transmembrane anion exchanger domain of AE1 is reported, which includes 14 trans Membrane helices and provides a basis for understanding the effects of mutations that lead to red blood cell diseases and also gives insight into the mechanism of ion transport.
Journal ArticleDOI
Band 3, the human red cell chloride/bicarbonate anion exchanger (AE1, SLC4A1), in a structural context.
Reinhart A. F. Reithmeier,Joseph R. Casey,Antreas C. Kalli,Mark S.P. Sansom,Yilmaz Alguel,So Iwata +5 more
TL;DR: The crystal structure of the dimeric membrane domain of human Band 3(1), the red cell chloride/bicarbonate anion exchanger 1 (AE1, SLC4A1), provides a structural context for over four decades of studies into this historic and important membrane glycoprotein.
Journal ArticleDOI
Recent advances in the selection and identification of antigen-specific nanobodies
TL;DR: The current state of the art in nanobody discovery is summarized, which can further boost the diverse applications of nanobodies in biomedicine and biotechnology.
Journal ArticleDOI
Molecular pathogenesis of Spondylocheirodysplastic Ehlers‐Danlos syndrome caused by mutant ZIP13 proteins
Bum-Ho Bin,Shintaro Hojyo,Toshiaki Hosaka,Jinhyuk Bhin,Hiroki Kano,Tomohiro Miyai,Mariko Ikeda,Tomomi Kimura-Someya,Mikako Shirouzu,Eun-Gyung Cho,Kazuhisa Fukue,Taiho Kambe,Wakana Ohashi,Kyu-Han Kim,Juyeon Seo,Dong-Hwa Choi,Yeon-Ju Nam,Daehee Hwang,Ayako Fukunaka,Yoshio Fujitani,Shigeyuki Yokoyama,Andrea Superti-Furga,Shiro Ikegawa,Tae Ryong Lee,Toshiyuki Fukada +24 more
TL;DR: The inhibition of degradation pathways rescued the protein expression levels, resulting in improved intracellular Zn homeostasis and further elucidation of these degradation processes may lead to novel therapeutic targets for SCD‐EDS.
Book ChapterDOI
The Dengue Virus Replication Complex: From RNA Replication to Protein-Protein Interactions to Evasion of Innate Immunity.
TL;DR: Structural and functional data are presented that augment the understanding of how viral RNA is replicated by dengue virus and lead to a working model that is captured in the form of a rather naïve "cartoon", which it is hoped will be refined towards an atomic model in the near future.
References
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TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Journal ArticleDOI
Crystal structure of the β2 adrenergic receptor-Gs protein complex.
Søren G. F. Rasmussen,Brian T. DeVree,Yaozhong Zou,Andrew C. Kruse,Ka Young Chung,Tong Sun Kobilka,Foon Sun Thian,Pil Seok Chae,Els Pardon,Els Pardon,Diane M. Calinski,Jesper Mosolff Mathiesen,Syed T. A. Shah,Joseph A. Lyons,Martin Caffrey,Samuel H. Gellman,Jan Steyaert,Jan Steyaert,Georgios Skiniotis,William I. Weis,Roger K. Sunahara,Brian K. Kobilka +21 more
TL;DR: This crystal structure represents the first high-resolution view of transmembrane signalling by a GPCR and the most surprising observation is a major displacement of the α-helical domain of Gαs relative to the Ras-like GTPase domain.
Journal ArticleDOI
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.
TL;DR: Here it is shown how the K+ channel displaces water molecules around an ion at its extracellular entryway, and how it holds a K+ ion in a square antiprism of water molecules in a cavity near its intracellular entry way.
Journal ArticleDOI
Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans.
TL;DR: The crystal structure at 2.8 Å resolution of the four protein subunits containing cytochrome c oxidase from the soil bacterium Paracoccus denitrificans, complexed with an antibody Fv fragment, is described and mechanisms for proton pumping are discussed.
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