Human intestinal microbiota composition is associated with local and systemic inflammation in obesity
Froukje J. Verdam,Susana Fuentes,Charlotte de Jonge,Erwin G. Zoetendal,Runi Erbil,Jan Greve,Wim A. Buurman,Willem M. de Vos,Sander S. Rensen +8 more
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TLDR
The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated and it was found that gut microbiota composition and inflammation are related to obesity.Abstract:
Objective
Intestinal microbiota have been suggested to contribute to the development of obesity, but the mechanism remains elusive. The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated.
Design and Methods
Fecal microbiota composition of 28 subjects (BMI 18.6-60.3 kg m−2) was analyzed by a phylogenetic profiling microarray. Fecal calprotectin and plasma C-reactive protein levels were determined to evaluate intestinal and systemic inflammation. Furthermore, HbA1c, and plasma levels of transaminases and lipids were analyzed. Gastroduodenal, small intestinal, and colonic permeability were assessed by a multisaccharide test.
Results
Based on microbiota composition, the study population segregated into two clusters with predominantly obese (15/19) or exclusively nonobese (9/9) subjects. Whereas intestinal permeability did not differ between clusters, the obese cluster showed reduced bacterial diversity, a decreased Bacteroidetes/Firmicutes ratio, and an increased abundance of potential proinflammatory Proteobacteria. Interestingly, fecal calprotectin was only detectable in subjects within the obese microbiota cluster (n = 8/19, P = 0.02). Plasma C-reactive protein was also increased in these subjects (P = 0.0005), and correlated with the Bacteroidetes/Firmicutes ratio (rs = −0.41, P = 0.03).
Conclusions
Intestinal microbiota alterations in obese subjects are associated with local and systemic inflammation, suggesting that the obesity-related microbiota composition has a proinflammatory effect.read more
Citations
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Book ChapterDOI
How to Manipulate the Microbiota: Fecal Microbiota Transplantation.
Susana Fuentes,Willem M. de Vos +1 more
TL;DR: Fecal microbiota transplantation is a rather straightforward therapy that manipulates the human gastrointestinal (GI) microbiota, by which a healthy donor microbiota is transferred into an existing but disturbed microbial ecosystem.
Journal ArticleDOI
Prebiotic dietary fibre intervention improves fecal markers related to inflammation in obese patients: results from the Food4Gut randomized placebo-controlled trial
Audrey M. Neyrinck,Julie Rodriguez,Zhengxiao Zhang,B. Seethaler,Cándido Robles Sánchez,Martin Roumain,Sophie Hiel,Laure B. Bindels,Patrice D. Cani,Nicolas Paquot,Miriam Cnop,Julie Anne Nazare,Martine Laville,Giulio G. Muccioli,Stephan C. Bischoff,Jens Walter,Jens Walter,Jean-Paul Thissen,Nathalie M. Delzenne +18 more
TL;DR: Inulin-type fructans (ITF) are prebiotic dietary fiber that may confer beneficial health effects, by interacting with the gut microbiota as mentioned in this paper, by promoting inulin intake versus placebo.
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Efficacy of an Anthocyanin and Prebiotic Blend on Intestinal Environment in Obese Male and Female Subjects
Shelly N. Hester,Angela Mastaloudis,Russell Gray,Joseph M. Antony,Malkanthi Evans,Steven M. Wood +5 more
TL;DR: The results suggest that regular consumption of the anthocyanin-prebiotic blend positively modulated the intestinal ecosystem and provided insights into the mechanisms of action and its impact on health benefits.
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Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations
Zora Djuric,Christine M. Bassis,Melissa A. Plegue,Jianwei Ren,Rena Chan,El Khansa Sidahmed,D. Kim Turgeon,Mack T. Ruffin,Ikuko Kato,Ananda Sen +9 more
TL;DR: The colonic mucosal bacterial community was associated with serum carotenoid concentrations at baseline but was not appreciably changed by dietary intervention, and the bacterial community in the Colonic mucosa was resistant to change after dietary intervention with either a Mediterranean diet or Healthy Eating diet.
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Enteric dysbiosis and fecal calprotectin expression in premature infants.
Thao Ho,Maureen Groer,Bradley Kane,Alyson L. Yee,Benjamin A. Torres,Jack A. Gilbert,Akhil Maheshwari +6 more
TL;DR: In premature infants, FC correlated with relative abundance of a specific pathobiont, Klebsiella, and not with that of the class Gammaproteobacteria, indicating a need to define dysbiosis at genera or higher levels of resolution.
References
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Fredrik Bäckhed,Hao Ding,Hao Ding,Ting Wang,Lora V. Hooper,Gou Young Koh,Andras Nagy,Clay F. Semenkovich,Jeffrey I. Gordon +8 more
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Journal ArticleDOI
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Junjie Qin,Yingrui Li,Zhiming Cai,Shenghui Li,Jianfeng Zhu,Fan Zhang,Suisha Liang,Wenwei Zhang,Yuanlin Guan,Dongqian Shen,Yangqing Peng,Dongya Zhang,Zhuye Jie,Wenxian Wu,Youwen Qin,Wenbin Xue,Junhua Li,Lingchuan Han,Donghui Lu,Peixian Wu,Yali Dai,Xiaojuan Sun,Zesong Li,Aifa Tang,Shilong Zhong,Xiaoping Li,Weineng Chen,Ran Xu,Mingbang Wang,Qiang Feng,Meihua Gong,Jing Yu,Yanyan Zhang,Ming Zhang,Torben Hansen,Gaston Sanchez,Jeroen Raes,Gwen Falony,Shujiro Okuda,Mathieu Almeida,Emmanuelle Le-chatelier,Pierre Renault,Nicolas Pons,Jean-Michel Batto,Zhaoxi Zhang,Hua Chen,Ruifu Yang,Wei-Mou Zheng,Songgang Li,Huanming Yang,Jian Wang,S. Dusko Ehrlich,Rasmus Nielsen,Oluf Pedersen,Oluf Pedersen,Karsten Kristiansen,Jun Wang +56 more
TL;DR: MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance.