Journal ArticleDOI
Animal models of human disease: zebrafish swim into view.
TLDR
This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.Abstract:
Despite the pre-eminence of the mouse in modelling human disease, several aspects of murine biology limit its routine use in large-scale genetic and therapeutic screening. Many researchers who are interested in an embryologically and genetically tractable disease model have now turned to zebrafish. Zebrafish biology allows ready access to all developmental stages, and the optical clarity of embryos and larvae allow real-time imaging of developing pathologies. Sophisticated mutagenesis and screening strategies on a large scale, and with an economy that is not possible in other vertebrate systems, have generated zebrafish models of a wide variety of human diseases. This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.read more
Citations
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Journal ArticleDOI
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
Yannick Doyon,Jasmine M. McCammon,Jeffrey C. Miller,Farhoud Faraji,Catherine Ngo,George E. Katibah,Rainier Amora,Toby Dylan Hocking,Lei Zhang,Edward J. Rebar,Philip D. Gregory,Fyodor D. Urnov,Fyodor D. Urnov,Sharon L. Amacher +13 more
TL;DR: The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
Journal ArticleDOI
In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish
TL;DR: Using a multicolor detection strategy, a spatiotemporal analysis of glycan expression and trafficking is performed and patterns that would be undetectable with conventional molecular imaging approaches are identified.
Journal ArticleDOI
Zebrafish embryos as an alternative to animal experiments--a commentary on the definition of the onset of protected life stages in animal welfare regulations.
Uwe Strähle,Stefan Scholz,Robert Geisler,Petra Greiner,Henner Hollert,Sepand Rastegar,Axel Schumacher,Ingrid W.T. Selderslaghs,Carsten Weiss,Hilda Witters,Thomas Braunbeck +10 more
TL;DR: It is concluded that zebrafish larvae can indeed be regarded as independently feeding from 120 h after fertilization, and should thus be subject to regulations for animal experiments from 120H after fertilized onwards.
Journal ArticleDOI
Preclinical Mouse Cancer Models: A Maze of Opportunities and Challenges
TL;DR: The current status, promise, and challenges of preclinical evaluation in advanced mouse cancer models are explored and emerging models for early-stage preclinical development are addressed.
Journal ArticleDOI
Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity.
TL;DR: New insights are provided into plastic particles' effects on zebrafish larvae, improving the understanding of their environmental risks to the aquatic environment and changing the larvae swimming behavior when co-exposed with EE2.
References
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Journal ArticleDOI
Effective targeted gene ‘knockdown’ in zebrafish
TL;DR: It is shown here that antisense, morpholino-modified oligonucleotides (morpholinos) are effective and specific translational inhibitors in zebrafish, and conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebra fish embryos.
Journal ArticleDOI
In vivo imaging of embryonic vascular development using transgenic zebrafish.
TL;DR: It is found that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis, and these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature.
Journal ArticleDOI
Heart Regeneration in Zebrafish
TL;DR: It is demonstrated histologically that zebrafish fully regenerate hearts within 2 months of 20% ventricular resection, showing that injury-induced cardiomyocyte proliferation in zebra fish can overcome scar formation, allowing cardiac muscle regeneration.
Journal ArticleDOI
The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio
P. Haffter,Michael Granato,Michael Brand,Mary C. Mullins,M. Hammerschmidt,D. A. Kane,J. Odenthal,F. J. M. Van Eeden,Y.-J. Jiang,Carl-Philipp Heisenberg,Robert N. Kelsh,Makoto Furutani-Seiki,Elisabeth Vogelsang,D. Beuchle,U. Schach,C. Fabian,Christiane Nüsslein-Volhard +16 more
TL;DR: It is estimated that the 372 genes defined by the mutants probably represent more than half of all genes that could have been discovered using the criteria of the screen, and the limits and the potentials of a genetic saturation screen in the zebrafish.
Journal ArticleDOI
Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
Adriana Donovan,Alison Brownlie,Alison Brownlie,Yi Zhou,Jennifer L. Shepard,Stephen J. Pratt,John Moynihan,Barry H. Paw,Anna Drejer,Bruce A. Barut,Bruce A. Barut,Agustín G. Zapata,Terence C. Law,Carlo Brugnara,Samuel E. Lux,Geraldine S. Pinkus,Jack L. Pinkus,Paul D. Kingsley,James Palis,Mark D. Fleming,Mark D. Fleming,Nancy C. Andrews,Nancy C. Andrews,Leonard I. Zon,Leonard I. Zon +24 more
TL;DR: The gene responsible for the hypochromic anaemia of the zebrafish mutant weissherbst is identified and Ferroportin1 function may be perturbed in mammalian disorders of iron deficiency or overload.
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