Journal ArticleDOI
Ingested double-stranded RNAs can act as species-specific insecticides.
TLDR
This is the first demonstration of RNAi following ingestion of dsRNA in all of the species tested, and the method offers promise of both higher throughput RNAi screens and the development of a new generation of species-specific insecticides.About:
This article is published in Insect Biochemistry and Molecular Biology.The article was published on 2009-11-01. It has received 542 citations till now. The article focuses on the topics: Manduca sexta & Drosophila.read more
Citations
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Journal ArticleDOI
RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Olle Terenius,Alexie Papanicolaou,Alexie Papanicolaou,Jennie S. Garbutt,Ioannis Eleftherianos,Hanneke Huvenne,Sriramana Kanginakudru,Merete Albrechtsen,Chunju An,Jean Luc Aymeric,Andrea Barthel,Piotr Bebas,Kavita Bitra,Alejandra Bravo,François Chevalier,Derek Collinge,Derek Collinge,Cristina M. Crava,Ruud A. de Maagd,Bernard Duvic,Martin A. Erlandson,Martin A. Erlandson,Ingrid Faye,G Felfoldi,Haruhiko Fujiwara,Ryo Futahashi,Ryo Futahashi,Archana S. Gandhe,H.S. Gatehouse,L. N. Gatehouse,Jadwiga M. Giebultowicz,Isabel Gómez,Cornelis J. P. Grimmelikhuijzen,Astrid T. Groot,Frank Hauser,David G. Heckel,Dwayne D. Hegedus,Dwayne D. Hegedus,Steven Hrycaj,Lihua Huang,J. Joe Hull,Kostas Iatrou,Masatoshi Iga,Michael R. Kanost,Joanna Kotwica,Changyou Li,Jianghong Li,Jisheng Liu,Magnus Lundmark,Shogo Matsumoto,Martina Meyering-Vos,Peter J. Millichap,Antónia Monteiro,Nirotpal Mrinal,Teruyuki Niimi,Daniela Nowara,Atsushi Ohnishi,Vicencio Oostra,Katsuhisa Ozaki,Maria P. Papakonstantinou,Aleksandar Popadic,Manchikatla Venkat Rajam,Suzanne V. Saenko,Robert M. Simpson,Mario Soberón,Michael R. Strand,Shuichiro Tomita,Umut Toprak,Ping Wang,Choon Wei Wee,Steven Whyard,Wenqing Zhang,Javaregowda Nagaraju,Richard H. ffrench-Constant,Salvador Herrero,Salvador Herrero,Karl H.J. Gordon,Luc Swevers,Guy Smagghe +78 more
TL;DR: Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity and that gene expression in epidermal tissues seems to be most difficult to silence.
Journal ArticleDOI
Characterizing the mechanism of action of double-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte).
Renata Bolognesi,Parthasarathy Ramaseshadri,Jerry Anderson,Pamela M. Bachman,William P. Clinton,Ronald Flannagan,Oliver Ilagan,Christina Lawrence,Steven L. Levine,William J. Moar,Geoffrey M. Mueller,Jianguo Tan,Joshua P. Uffman,B. Elizabeth Wiggins,Gregory R. Heck,Gerrit Segers +15 more
TL;DR: These events (dsRNA uptake, target mRNA and protein suppression, systemic spreading, growth inhibition and eventual mortality) comprise the overall mechanism of action by which DvSnf7 dsRNA affects WCR via oral delivery and provides insights as to how targeted dsRNAs in general are active against insects.
Journal ArticleDOI
Towards the elements of successful insect RNAi.
Jeffrey G. Scott,Kristin Michel,Lyric C. Bartholomay,Blair D. Siegfried,Wayne B. Hunter,Guy Smagghe,Kun Yan Zhu,Angela E. Douglas +7 more
TL;DR: An enhanced conceptual understanding ofRNAi function in insects will facilitate the application of RNAi for dissection of gene function, and to fast-track the application to both control pests and develop effective methods to protect beneficial insects and non-insect arthropods from viral and parasitic diseases.
Journal ArticleDOI
Silencing of Aphid Genes by dsRNA Feeding from Plants
Marco Pitino,Alexander D. Coleman,Massimo E. Maffei,Christopher J. Ridout,Saskia A. Hogenhout +4 more
TL;DR: The developed plant-mediated RNAi technology for aphids to allow for gene silencing in the aphid natural environment and minimize handling of these insects during experiments demonstrates the feasibility of the plant- mediated RNAi approach for aphid control.
Journal ArticleDOI
Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata.
TL;DR: This study provides the first example of an effective RNAi response in insects after feeding ds RNA produced in bacteria, and suggests that the efficient induction of RNAi using bacteria to deliver dsRNA is a possible method for management of Colorado potato beetle.
References
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Journal ArticleDOI
A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
Journal ArticleDOI
Origins and Mechanisms of miRNAs and siRNAs
TL;DR: This work has revealed unexpected diversity in their biogenesis pathways and the regulatory mechanisms that they access, which has direct implications for fundamental biology as well as disease etiology and treatment.
Journal Article
RNA interference : RNA
TL;DR: A conserved biological response to double-stranded RNA, known variously as RNA interference (RNAi) or post-transcriptional gene silencing, mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
Journal ArticleDOI
Specific interference by ingested dsRNA
Lisa Timmons,Andrew Fire +1 more
TL;DR: This work shows that C. elegans can respond in a gene-specific manner to dsRNA encountered in the environment, and finds that Escherichia coli bacteria expressing dsRNAs can confer specific interference effects on the nematode larvae that feed on them.
Journal ArticleDOI
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
TL;DR: An efficient induction of RNAi using bacteria to deliver double-stranded RNA is reported, found to be most effective in inducing RNAi for non-neuronal tissue of late larval and adult hermaphrodites, with decreased effectiveness in the nervous system, in early larval stages, and in males.
Related Papers (5)
Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review.
Hanneke Huvenne,Guy Smagghe +1 more
RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Olle Terenius,Alexie Papanicolaou,Alexie Papanicolaou,Jennie S. Garbutt,Ioannis Eleftherianos,Hanneke Huvenne,Sriramana Kanginakudru,Merete Albrechtsen,Chunju An,Jean Luc Aymeric,Andrea Barthel,Piotr Bebas,Kavita Bitra,Alejandra Bravo,François Chevalier,Derek Collinge,Derek Collinge,Cristina M. Crava,Ruud A. de Maagd,Bernard Duvic,Martin A. Erlandson,Martin A. Erlandson,Ingrid Faye,G Felfoldi,Haruhiko Fujiwara,Ryo Futahashi,Ryo Futahashi,Archana S. Gandhe,H.S. Gatehouse,L. N. Gatehouse,Jadwiga M. Giebultowicz,Isabel Gómez,Cornelis J. P. Grimmelikhuijzen,Astrid T. Groot,Frank Hauser,David G. Heckel,Dwayne D. Hegedus,Dwayne D. Hegedus,Steven Hrycaj,Lihua Huang,J. Joe Hull,Kostas Iatrou,Masatoshi Iga,Michael R. Kanost,Joanna Kotwica,Changyou Li,Jianghong Li,Jisheng Liu,Magnus Lundmark,Shogo Matsumoto,Martina Meyering-Vos,Peter J. Millichap,Antónia Monteiro,Nirotpal Mrinal,Teruyuki Niimi,Daniela Nowara,Atsushi Ohnishi,Vicencio Oostra,Katsuhisa Ozaki,Maria P. Papakonstantinou,Aleksandar Popadic,Manchikatla Venkat Rajam,Suzanne V. Saenko,Robert M. Simpson,Mario Soberón,Michael R. Strand,Shuichiro Tomita,Umut Toprak,Ping Wang,Choon Wei Wee,Steven Whyard,Wenqing Zhang,Javaregowda Nagaraju,Richard H. ffrench-Constant,Salvador Herrero,Salvador Herrero,Karl H.J. Gordon,Luc Swevers,Guy Smagghe +78 more