RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Olle Terenius,Alexie Papanicolaou,Alexie Papanicolaou,Jennie S. Garbutt,Ioannis Eleftherianos,Hanneke Huvenne,Sriramana Kanginakudru,Merete Albrechtsen,Chunju An,Jean Luc Aymeric,Andrea Barthel,Piotr Bebas,Kavita Bitra,Alejandra Bravo,François Chevalier,Derek Collinge,Derek Collinge,Cristina M. Crava,Ruud A. de Maagd,Bernard Duvic,Martin A. Erlandson,Martin A. Erlandson,Ingrid Faye,G Felfoldi,Haruhiko Fujiwara,Ryo Futahashi,Ryo Futahashi,Archana S. Gandhe,H.S. Gatehouse,L. N. Gatehouse,Jadwiga M. Giebultowicz,Isabel Gómez,Cornelis J. P. Grimmelikhuijzen,Astrid T. Groot,Frank Hauser,David G. Heckel,Dwayne D. Hegedus,Dwayne D. Hegedus,Steven Hrycaj,Lihua Huang,J. Joe Hull,Kostas Iatrou,Masatoshi Iga,Michael R. Kanost,Joanna Kotwica,Changyou Li,Jianghong Li,Jisheng Liu,Magnus Lundmark,Shogo Matsumoto,Martina Meyering-Vos,Peter J. Millichap,Antónia Monteiro,Nirotpal Mrinal,Teruyuki Niimi,Daniela Nowara,Atsushi Ohnishi,Vicencio Oostra,Katsuhisa Ozaki,Maria P. Papakonstantinou,Aleksandar Popadic,Manchikatla Venkat Rajam,Suzanne V. Saenko,Robert M. Simpson,Mario Soberón,Michael R. Strand,Shuichiro Tomita,Umut Toprak,Ping Wang,Choon Wei Wee,Steven Whyard,Wenqing Zhang,Javaregowda Nagaraju,Richard H. ffrench-Constant,Salvador Herrero,Salvador Herrero,Karl H.J. Gordon,Luc Swevers,Guy Smagghe +78 more
TLDR
Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity and that gene expression in epidermal tissues seems to be most difficult to silence.About:
This article is published in Journal of Insect Physiology.The article was published on 2011-02-01 and is currently open access. It has received 698 citations till now. The article focuses on the topics: RNA interference & RNA silencing.read more
Citations
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The monarch butterfly genome yields insights into long-distance migration
TL;DR: The monarch genome reveals a vertebrate-like opsin whose existence in insects is widespread; a full repertoire of molecular components for the monarch circadian clockwork; all members of the juvenile hormone biosynthetic pathway whose regulation shows unexpected sexual dimorphism; and a variant of the sodium/potassium pump that underlies a valuable chemical defense mechanism.
Journal ArticleDOI
Characterizing the mechanism of action of double-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte).
Renata Bolognesi,Parthasarathy Ramaseshadri,Jerry Anderson,Pamela M. Bachman,William P. Clinton,Ronald Flannagan,Oliver Ilagan,Christina Lawrence,Steven L. Levine,William J. Moar,Geoffrey M. Mueller,Jianguo Tan,Joshua P. Uffman,B. Elizabeth Wiggins,Gregory R. Heck,Gerrit Segers +15 more
TL;DR: These events (dsRNA uptake, target mRNA and protein suppression, systemic spreading, growth inhibition and eventual mortality) comprise the overall mechanism of action by which DvSnf7 dsRNA affects WCR via oral delivery and provides insights as to how targeted dsRNAs in general are active against insects.
Journal ArticleDOI
Towards the elements of successful insect RNAi.
Jeffrey G. Scott,Kristin Michel,Lyric C. Bartholomay,Blair D. Siegfried,Wayne B. Hunter,Guy Smagghe,Kun Yan Zhu,Angela E. Douglas +7 more
TL;DR: An enhanced conceptual understanding ofRNAi function in insects will facilitate the application of RNAi for dissection of gene function, and to fast-track the application to both control pests and develop effective methods to protect beneficial insects and non-insect arthropods from viral and parasitic diseases.
Journal ArticleDOI
RNAi efficiency, systemic properties, and novel delivery methods for pest insect control : what we know so far
TL;DR: Important implications and possibilities to increase RNAi efficiency by delivery of dsRNA through non-transformative methods are explored, including delivery by symbionts, plant viruses, trunk injections, root soaking, and transplastomic plants.
Journal ArticleDOI
Delivery of dsRNA for RNAi in insects: an overview and future directions
Na Yu,Olivier Christiaens,Jisheng Liu,Jinzhi Niu,Kaat Cappelle,Silvia Caccia,Hanneke Huvenne,Guy Smagghe +7 more
TL;DR: The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.
References
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Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
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MicroRNA genes are transcribed by RNA polymerase II.
TL;DR: The first direct evidence that miRNA genes are transcribed by RNA polymerase II (pol II) is presented and the detailed structure of a miRNA gene is described, for the first time, by determining the promoter and the terminator of mir‐23a∼27a‐24‐2.
Journal Article
RNA interference : RNA
TL;DR: A conserved biological response to double-stranded RNA, known variously as RNA interference (RNAi) or post-transcriptional gene silencing, mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
Journal ArticleDOI
Mechanisms of gene silencing by double-stranded RNA
Gunter Meister,Thomas Tuschl +1 more
TL;DR: A key step in known silencing pathways is the processing of dsRNAs into short RNA duplexes of characteristic size and structure, which guide RNA silencing by specific and distinct mechanisms.
Journal ArticleDOI
RNA silencing in plants
TL;DR: Diverse biological roles of these pathways have been established, including defence against viruses, regulation of gene expression and the condensation of chromatin into heterochromatin, and the full extent of this functional diversity in genetic and epigenetic mechanisms of genome control is investigated.
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