Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Virus discovery and human coronavirus NL63
TL;DR: This dissertation aims to provide a history of web exceptionalism from 1989 to 2002, a period chosen in order to explore its roots as well as specific cases up to and including the year in which descriptions of “Web 2.0” began to circulate.
Dissertation
Utilisation des cellules souches induites à la pluripotence pour la modélisation pathologique du syndrome de Hutchinson Gilford
TL;DR: In this article, the authors present a set of mecanismes and moleculaires for the development of therapeutiques for the progression of the maladie and the maturation of the proteine mutee.
Dissertation
The role of genetic factors in neuronal migration disorders and epilepsy
TL;DR: A Brazilian family with a novel idiopathic autosomal dominant partial epilepsy with Peri-central Spikes is studied and the role of genes involved in neuronal migration has been studied in a model system, the zebrafish, providing the model system for the study of the action of this gene.
Molecular mechanisms that govern the establishment of sensory-motor networks
TL;DR: Validation and characterization of the expression patterns of candidate genes previously predicted by a whole-genome expression analysis of differentially projecting sensory and motor neurons further contributes to the decipherment of molecular mechanisms that govern sensory-motor network formation.
Journal ArticleDOI
Kynurenine-3-monooxygenase expression is activated in the pancreatic endocrine cells by diabetes and its blockade improves glucose-stimulated insulin secretion.
Junjun Liu,Danielle Bailbe,S. Raynal,C. Carbonne,Delong Zhen,Julien Dairou,Blandine Gausserès,Mathieu Pierre Jean Armanet,Thomas Domet,Caterina Luana Pitasi,Jamileh Movassat,Chai K. Lim,Gilles J. Guillemin,V. Autier,Micheline Kergoat,Bernard Portha +15 more
TL;DR: In this paper , the authors examined the TKP enzymes expression in diabetic GK islets, and the glucose-stimulated insulin secretion (GSIS) after KMO knockdown using specific morpholino-oligonucleotides against KMO or KMO blockade using the specific inhibitor Ro618048 in GK rats.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.