Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
Reads0
Chats0
TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
More filters
Dissertation
Thermodynamics and kinetics of antisense oligonucleotide hybridization to a structured mRNA target
TL;DR: Thesis (Sc. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, February 2002.
Dissertation
Investigating the Role of Synapsin II in Neurological Disorders Involving Dysregulated Dopaminergic Transmission
TL;DR: Treatment with atypical APDs significantly increases synapsin II in the prefrontal cortex (PFC) of patients, which may underlie the beneficial effects of these drugs, and investigates the expression of various presynaptic proteins in post-mortem samples from patients with Parkinson's disease.
The role of ovine betaretroviruses in uteroplacental function
TL;DR: Results demonstrate that alterations in expression of enJSRVs and HYAL2 in the sheep uterus and placenta suggest the probability of a variety of physiological roles in implantation and Placenta.
Dissertation
Alanina aminotransferasa en Sparus aurata: control de la expresión génica mediante RNAi y de la actividad enzimática por aminooxiacetato
González García,Juan Diego +1 more
TL;DR: Nuestros estudios muestran that the inyeccion intraperitoneal de doradas con nanoparticulas del complejo pCpG-siRNA-quitosan resulto adecuada para promover the expresion of un siRNA para bloquear the expresso of cALT1 en higado de Sparus aurata.
Morpholino Treatment Improved Muscle Function and Pathology in Pitx1 Transgenic Mice
TL;DR: In this paper, a tetrepressible muscle-specific PITX1 transgenic mouse model was generated, which developed phenotypes of muscular dystrophy after the paired-like homeodomain transcription factor 1 (PITX1) was induced.
References
More filters
Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.