Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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Journal ArticleDOI
Docking protein SNT1 is a critical mediator of fibroblast growth factor signaling during Xenopus embryonic development.
TL;DR: It is shown that the Xenopus homolog of mammalian SNT1/FRS‐2 (XSNT1) plays a critical role in the appropriate formation of mesoderm‐derived tissue during embryogenesis and is required for early Xenopus development.
Journal ArticleDOI
Hoogsteen-Paired Homopurine [RP-PS]-DNA and Homopyrimidine RNA Strands Form a Thermally Stable Parallel Duplex
TL;DR: This work has shown that homopurine deoxyribonucleoside phosphorothioates possessing all internucleotide linkages of R(P) configuration form a duplex with an RNA or 2'-OMe-RNA strand with Hoogsteen complementarity that is thermally more stable than parallelHoogsteen duplexes and antiparallel Watson-Crick dupLexes formed by unmodified homopURine DNA molecules of the same sequence with corresponding RNA templates.
Patent
Molecule for treating an inflammatory disorder
Ommen Garrit-Jan Boudewijn Van,A. Aartsma-Rus,Deutekom Judith Christina Theodora Van,Kimpe Josephus Johannes De,Joseph Stephan Verbeek,Aliye Seda Yilmaz-Elis +5 more
TL;DR: In this article, two types of oligonucleotides for treating an inflammatory disorder: an oligonotide which is able of altering the splicing of a pre-mRNA encoding a C5 in order to decrease the amount of C5a, and an oligoneotide which was able of changing the splice of an IL-1RAcP encoding a R-1RPCP to increase the number of soluble IL- 1RAcPs.
Patent
Compound and method for treating androgen-independent prostate cancer
TL;DR: In this article, a morpholino antisense compound having uncharged phosphorus-containing backbone linkages and a base sequence that is complementary to a target region containing at least 12 contiguous bases in a preprocessed or processed human androgen receptor transcript was designed for treating prostate cancer in a subject having a hormone-refractory (androgen-independent) prostate cancer.
Physiological Role in Regulation of Ependymal Ciliary Function and Characteristics in Human Female Reproductive Tissues
TL;DR: Gai2 has a cilia-related physiological role in rat brain and it is hormonally regulated in the human female reproductive tract, with specific enrichment in the Fallopian tube cilia and endometrial glands.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.