Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Patent
Compositions and methods for modulating hemoglobin gene family expression
TL;DR: In this article, single stranded oligonucleotides for activating or enhancing expression of hemoglobin genes (HBB, HBD, HBEl, HBG1 or HBG2) are presented.
Book ChapterDOI
Spatiotemporal control of embryonic gene expression using caged morpholinos.
TL;DR: This chapter describes general principles for cMO design and methods for c MO assembly in three steps from commercially available reagents and demonstrates how cMOs can be effective tools for functional genomic studies in zebrafish and other model organisms.
Journal ArticleDOI
Enhanced activity of antisense phosphorothioate oligos against Leishmania amastigotes: augmented uptake of oligo, ribonuclease H activation, and efficient target intervention under altered growth conditions
TL;DR: Enhanced uptake of S-oligos at the acidic pH of macrophage phagolysosomes and activation of RNase H may explain the efficient killing of the parasite in macrophages, both in tissue culture and in the animal model, by antisense miniexon oligonucleotide/PLL.
Journal ArticleDOI
Cardiosulfa induces heart deformation in zebrafish through the AhR-mediated, CYP1A-independent pathway.
Sung Kyun Ko,Injae Shin +1 more
TL;DR: The results of the present study show that cardiosulfa‐promoted heart deformation is protected by negative regulators of the aryl hydrocarbon receptor (AhR) signaling pathway, such as the AhR antagonist CH‐223191 and an AhR2‐morpholino antisense oligonucleotide, zfahr2‐MO, but the toxic effect of cardios sulfura is not alleviated by zfcyp1a‐MO.
Journal ArticleDOI
Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in Plasmodium falciparum
Aprajita Garg,Donna Wesolowski,Dulce Alonso,Kirk W. Deitsch,Choukri Ben Mamoun,Sidney Altman +5 more
TL;DR: Evidence for successful use of morpholino oligomers (MO) to mediate degradation of target mRNAs or to inhibit RNA splicing or translation of several genes of P. falciparum involved in chloroquine transport, apicoplast biogenesis, and phospholipid biosynthesis is presented.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.