Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Dissertation
The effect of hypoxia on alternative splicing in prostate cancer cell lines
TL;DR: Evidence is provided that alterations in alternative splicing patterns of key genes is one method tumour cells adapt to hypoxia, and in contrast to previous research CLK1 was found to be localised to the cytoplasm in both normoxia andHypoxia.
Journal ArticleDOI
Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio)
TL;DR: The expression pattern, protein localisation and gross anatomical consequences of depleting invadolysin in the teleost Danio rerio are examined to highlight a common feature shared by, and a requirement in, these distinct morphological events dependent on cell migration.
Book ChapterDOI
Antisense-mediated exon-skipping to induce gene knockdown.
Petra Disterer,Bernard Khoo +1 more
TL;DR: Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform as discussed by the authors. But this method is not suitable for gene expression.
Journal ArticleDOI
Ligand-based backbone modifications for metal-chelating nucleic acids
TL;DR: Ligands were incorporated into the backbone of DNA for nucleoside replacements, and the binding of metal ions, such as Cu2+, Pt2+ and Pd4+, was shown to influence stability of the resulting duplexes.
Investigating the role of retinoblastoma-binding protein 9 in human pluripotent stem cells and embryonic development
TL;DR: The present work focuses on hiPSCs: advancements in cell reprogramming and disease modelling, and core regulatory factors controlling pluripotency in hESCs, a model of embryonic development based on zebrafish embryogenesis.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.