Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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Journal ArticleDOI
Effective intracellular delivery of oligonucleotides in order to make sense of antisense.
Fuxin Shi,Dick Hoekstra +1 more
TL;DR: Evidence is accumulating which suggests that by modulating the surface properties of the carrier, the kinetics of such changes can be controlled, thus providing possibilities for programmable release of the carriers' carrier contents.
Journal ArticleDOI
Nanotechnologies and controlled release systems for the delivery of antisense oligonucleotides and small interfering RNA.
Elias Fattal,Gillian Barratt +1 more
TL;DR: Progress is described that has been made using delivery systems composed of natural or synthetic polymers in the form of complexes, nanoparticles or microparticles within nano‐ and microsized drug delivery systems.
Patent
Trpm-2 antisense therapy
TL;DR: In this paper, it has been determined that antisense therapy which reduces the expression of TRPM-2 provides therapeutic benefits in the treatment of cancer, in particular, in prostate cancer and renal cell cancer.
Journal ArticleDOI
Versatile synthesis and rational design of caged morpholinos.
Xiaohu Ouyang,Ilya A. Shestopalov,Surajit Sinha,Genhua Zheng,Cameron L W Pitt,Wen Hong Li,Andrew Olson,James K. Chen +7 more
TL;DR: Optimize methods for the design and synthesis of hairpin cMOs incorporating a dimethoxynitrobenzyl (DMNB)-based bifunctional linker that permits cMO assembly in only three steps from commercially available reagents establish the generality of cMO technologies and will facilitate the application of these chemical probes in vivo for functional genomic studies.
Journal ArticleDOI
Endo-Porter: A Novel Reagent for Safe, Effective Delivery of Substances into Cells
TL;DR: The basic design strategy used to develop Endo‐Porter is described, test systems used to guide that development, and the effects of various structural parameters, including size and composition of the lipophilic face, size and compositions of the weak‐base face, andThe relationship between peptide length and delivery efficiency in the presence of serum is described.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.