Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Book ChapterDOI
Vascular endothelial growth factor and its receptors in embryonic zebrafish blood vessel development.
TL;DR: The zebra fish vasculature, zebrafish V EGF and VEGF receptors, advantages of the zebra Fish model, resources, and methods of determining growth factor and receptor function are described.
PatentDOI
Morpholino-mediated increase in soluble Flt-1 expression results in decreased ocular and tumor neovascularization
TL;DR: In this article, a method of inhibiting angiogenesis in a subject can include binding an antisense morpholino to an mRNA splicing site of VEGFR1 selected from exon13_intron13 junction, intron13_exon14 junction, or a combination thereof.
Journal ArticleDOI
Targeted knockdown of an opsin gene inhibits the swimming behaviour photoresponse of ascidian larvae
TL;DR: It is shown that antisense morpholinos (MOs) are effective and specific translational inhibitors in C. intestinalis larvae and the inhibition of the photic behaviour of the knockdown larvae is solely due to the suppression of the synthesis of the visual pigments in the photoreceptors.
Journal ArticleDOI
Antisense therapy in malignant diseases: status quo and quo vadis?
TL;DR: In this review, therapeutic concepts, clinical studies and new promising molecular targets to treat malignancies with AS ODNs are summarized.
Journal ArticleDOI
Androgen receptor down-regulation in prostate cancer with phosphorodiamidate morpholino antisense oligomers
Yoo-Joung Ko,Gayathri R. Devi,Carla A. London,Anthony Kayas,Muralimohan T. Reddy,Patrick L. Iversen,Glenn J. Bubley,Steven P. Balk +7 more
TL;DR: The reduction of AR protein with an antisense PMO may be an effective method of interfering with AR mediated growth in advanced human PCa.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.