Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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MicroRNAs in the Tumor Biology of Soft Tissue Sarcomas
TL;DR: It is shown that microRNAs can discriminate diverse liposarcoma subtype, lipomas and normal fat and fine-tune the expression of HIF3α in hypoxic tumor cells, and it is demonstrated that hypoxia inducible micro RNAs, miR-210 andMiR-485-5p, fine-Tune theexpression of Hif3 α in hyp toxic tumor cells.
Book ChapterDOI
Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments
Christine Di Cresce,Christine Di Cresce,Colin Way,Colin Way,Mateusz Rytelewski,Mateusz Rytelewski,Saman Maleki Vareki,Saman Maleki Vareki,Supritha Nilam,Mark Vincent,Mark Vincent,James Koropatnick,James Koropatnick,Peter J. Ferguson,Peter J. Ferguson +14 more
TL;DR: Progress in developing antisense drugs to downregulate genes mediating malignant characteristics in tumors originating in multiple tissues is reviewed, with single-stranded DNA ASOs currently the most advanced in clinical testing.
Journal ArticleDOI
Leptin-a mediates transcription of genes that participate in central endocrine and phosphatidylinositol signaling pathways in 72-hour embryonic zebrafish (Danio rerio).
TL;DR: A role for zebrafish Leptin-a in influencing expression of genes that mediate phosphatidylinositol and central endocrine signaling is presented.
Patent
The rna interactome of polycomb repressive complex 1 (prc1)
TL;DR: In this paper, the authors describe polycomb-associated RNAs, libraries and fragments of them, inhibitory nucleic acids and methods and compositions for targeting RNAs and methods of use thereof.
Book ChapterDOI
Studying disorders of vertebrate iron and heme metabolism using zebrafish.
TL;DR: This chapter first reviews well-established methods, such as large-scale mutagenesis screens that have led to the initial identification of a series of iron and heme transporters and the generation of a variety of mutant lines, and highlights several recently developed approaches.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.