Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
Reads0
Chats0
TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
More filters
Journal ArticleDOI
WDDD: Worm Developmental Dynamics Database
Koji Kyoda,Eru Adachi,Eriko Masuda,Yoko Nagai,Yoko Suzuki,Taeko Oguro,Mitsuru Urai,Ryoko Arai,Mari Furukawa,Kumiko Shimada,Junko Kuramochi,Eriko Nagai,Shuichi Onami +12 more
TL;DR: A database, the Worm Developmental Dynamics Database, is created, which stores a collection of quantitative information about cell division dynamics in early Caenorhabditis elegans embryos with single genes silenced by RNA-mediated interference.
Patent
Methods and means for efficient skipping of at least one of the following exons of the human Duchenne muscular dystrophy gene: 43, 46, 50- 53.
Josephus Johannes De Kimpe,Gerardus Johannes Platenburg,Judith Christina Theodora Van Deutekom,Annemieke Aartsma-Rus,Garrit-Jan Boudewijn Van Ommen +4 more
TL;DR: In this paper, a method for inducing skipping of at least one of exon 43, exon 46, exons 50-53 of the DMD pre- mRNA in a patient, was described.
Journal ArticleDOI
Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation.
Tadashi Wada,Masatoshi Hara,Takuya Taneda,Cao Qingfu,Ryouhei Takata,Kanako Moro,Kei Takeda,Takeo Kishimoto,Hiroshi Handa +8 more
TL;DR: An antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstream from a poly(A) tail junction in the 3′-untranslated region (UTR) of maternal mRNA to targeted downregulation of maternal mRNAs in animal oocytes, eggs and early embryos.
Journal ArticleDOI
Vascular endothelial growth factor receptor 1 morpholino increases graft survival in a murine penetrating keratoplasty model
Yang Kyung Cho,Xiaohui Zhang,Hironori Uehara,Jason R. Young,Bonnie Archer,Balamurali K. Ambati +5 more
TL;DR: VEGFR1_MO decreased angiogenesis and lymphangiogenesis, resulting in increased graft survival in both NR PK and HR PK, and this beneficial effect is synergistically enhanced with steroid treatment in HR PK.
References
More filters
Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.