Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Vascular Endothelial Growth Factor Receptor 1 Morpholino Decreases Angiogenesis in a Murine Corneal Suture Model
Yang Kyung Cho,Yang Kyung Cho,Hironori Uehara,Jason R. Young,Bonnie Archer,Xiaohui Zhang,Balamurali K. Ambati +6 more
TL;DR: Ft morpholinos decrease mFlt-1 and increase sFlt,1 levels, resulting in decreased neovascularization in a murine corneal suture model, thereby limiting angiogenesis and inflammation in a mouse cornea injury model.
Journal ArticleDOI
Synthesis of Phosphorodiamidate Morpholino Oligonucleotides and Their Chimeras Using Phosphoramidite Chemistry
TL;DR: Treatment of HeLa cells with fluorescently labeled PMO chimeras demonstrated that these analogues were efficiently taken up by cells in the presence of a lipid transfection reagent and should open new pathways for research into the antisense, diagnostic, and nanotechnology oligonucleotide fields.
Journal ArticleDOI
BMP- and neuropilin 1-mediated motor axon navigation relies on spastin alternative translation
Nicolas Jardin,François Giudicelli,Daniel Ten Martin,Anaïs Vitrac,Stéphanie De Gois,Rachel Allison,Corinne Houart,Evan Reid,Jamilé Hazan,Coralie Fassier +9 more
TL;DR: It is suggested that defective BMP and neuropilin 1 signalling may contribute to the motor phenotype in a vertebrate model of spastin depletion.
Journal ArticleDOI
Biomaterials for polynucleotide delivery to anchorage-independent cells.
TL;DR: This article summarizes the techniques and biomaterials that have been utilized for transfection of anchorage-independent cells and discusses the structure-activity relationship of various biomaterial carriers of polynucleotides.
Journal ArticleDOI
Synthesis of phosphorodiamidate morpholino oligonucleotides by H-phosphonate method
TL;DR: In this paper, the synthesis of T-containing morpholino oligomers by H-phosphonate method on solid support has been reported for the first time, where the phosphonamidate backbone was oxidized by iodine-dimethylamine mixture followed by the cleavage from solid support by aqueous ammonia to obtain 15-mer T -containing PMO.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.