Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Alx1, a member of the Cart1/Alx3/Alx4 subfamily of Paired-class homeodomain proteins, is an essential component of the gene network controlling skeletogenic fate specification in the sea urchin embryo.
TL;DR: It is shown that the ancestral deuterostome had a population of biomineral-forming mesenchyme cells that expressed an Alx1-like protein, which is highly conserved among euechinoid sea urchins and is closely related to the Cart1/Alx3/AlX4 family of vertebrate homeodomain proteins.
Journal ArticleDOI
Endosome trapping limits the efficiency of splicing correction by PNA-oligolysine conjugates.
TL;DR: The conjugation of short oligolysine tails has been used to deliver a correcting peptide nucleic acid (PNA) sequence in a positive readout assay in which ON hybridization to the cryptic splice site is strictly required for the expression of a luciferase reporter gene.
Journal ArticleDOI
FGF signalling controls formation of the apical sensory organ in the cnidarian Nematostella vectensis.
TL;DR: It is proposed that the control of the development of sensory structures at the aboral pole of ciliated larvae is an ancestral function of FGF signalling, and it is shown that loss of the apical organ upon NvFGFa1 knockdown blocks metamorphosis into polyps.
Journal ArticleDOI
Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA.
Elias Fattal,Amélie Bochot +1 more
TL;DR: The intraocular administration of nucleic acids offers interesting perspectives for the treatment of ocular diseases and the siRNAs appear very promising as they are very potent inhibitors of protein expression.
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The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning
Deborah M. Kurrasch,Clement C. Cheung,Florence Y. Lee,Phu V. Tran,Kenji Hata,Holly A. Ingraham +5 more
TL;DR: This study identifies ∼200 genes highly enriched in neonatal (postnatal day 0) mouse VMH tissue and provides a novel molecular toolkit to probe further the genetic basis of innate neuroendocrine behavioral responses.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.