Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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Journal ArticleDOI
Using sequence-specific oligonucleotides to inhibit bacterial rRNA.
TL;DR: Nonconventional approaches to screening for ribosome inhibition and function of particular rRNA regions are described, including inhibitors against rRNA that may be designed according to nucleotide sequence and higher order structure.
Journal ArticleDOI
Effective delivery of antisense peptide nucleic acid oligomers into cells by anthrax protective antigen.
TL;DR: Protectiveantigen (PA, a non-toxic component of anthrax toxin) could transport antisense PNA oligomers into reporter cells that contain luciferase transgenes with mutant beta-globin IVS2 intronic inserts, which permit aberrant pre-mRNA splicing and impair Luciferase expression.
Journal ArticleDOI
Use of Antisense Oligonucleotides: Advantages, Controls, and Cardiovascular Tissue
TL;DR: This review highlights some of the advances made in the field of antisense research, placing an emphasis on uses and proper controls.
Journal ArticleDOI
AMOD: a morpholino oligonucleotide selection tool
Eric W. Klee,Kyong Jin Shim,Michael A. Pickart,Michael A. Pickart,Stephen C. Ekker,Lynda B. M. Ellis +5 more
TL;DR: AMOD is a web-based program that aids in the functional evaluation of nucleotide sequences through sequence characterization and antisense morpholino oligonucleotide (target site) selection and has been used at the University of Minnesota to design ∼200 morpholinos for a functional genomics screen in zebrafish.
Journal ArticleDOI
Induced sterility in fish and its potential and challenges for aquaculture and germ cell transplantation technology: A review
Amin Golpour,Mohammad Abdul Momin Siddique,Diógenes Henrique de Siqueira-Silva,Martin Pšenička +3 more
TL;DR: A review of currently used technologies and recent advances in induction of sterility in fish, especially those intended for use in germ cell transplantation, and the implications of these approaches remains incomplete.
References
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Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
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Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
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Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.