Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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Journal ArticleDOI
Inhibition of replication and transcription activator and latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus by morpholino oligomers
Yan-Jin Zhang,Kai-Yu Wang,David A. Stein,Deendayal Patel,Rheba Watkins,Hong M. Moulton,Patrick L. Iversen,David O. Matson +7 more
TL;DR: Results suggest that RP1 P-PMO can specifically block KSHV replication, and further study is warranted.
Journal ArticleDOI
Oligonucleotide-based strategies to reduce gene expression.
John M. Dagle,Daniel L. Weeks +1 more
TL;DR: Oligonucleotide-based approaches offer a variety of means to potentially disrupt normal gene expression and the basis for some of these approaches is presented in this review.
Journal ArticleDOI
Xenopus frizzled-7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation.
Saulius Sumanas,Stephen C. Ekker +1 more
TL;DR: The results presented here confirm the previous findings that Xfz7 is required for the dorsoventral Wnt/b-catenin axis induction pathway and demonstrate a XfZ7 requirement in a zygotic Wnt pathway that regulates morphogenetic movements during gastrulation.
Journal ArticleDOI
Functional Genomics Tools for the Analysis of Zebrafish Pigment
Michael A. Pickart,Sridhar Sivasubbu,Aubrey L. Nielsen,Sabitha Shriram,Richard A. King,Stephen C. Ekker +5 more
TL;DR: The zebrafish has a number of characteristics that make it an especially valuable model for the study of pigment cell biology and disease and an ongoing, reverse-genetic, MO-based screen for the rapid identification of gene function promises to be a valuable complement to other high-throughput microarray and proteomic approaches for understanding pigmentcell biology.
Journal ArticleDOI
Inhibition of respiratory syncytial virus infections with morpholino oligomers in cell cultures and in mice.
Shen-Hao Lai,Shen-Hao Lai,Shen-Hao Lai,David A. Stein,Antonieta Guerrero-Plata,Sui Ling Liao,Sui Ling Liao,Sui Ling Liao,Teodora Ivanciuc,Chao Hong,Patrick L. Iversen,Antonella Casola,Roberto P. Garofalo +12 more
TL;DR: Data show that the AUG-2 PPMO possesses potent anti-RSV activity and is worthy of further investigation as a candidate for potential therapeutic application.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.