Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
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Journal ArticleDOI
A serotonin receptor antagonist induces oocyte maturation in both frogs and mice: evidence that the same G protein-coupled receptor is responsible for maintaining meiosis arrest in both species.
Yinglun Sheng,Yinglun Sheng,Ling Wang,Ling Wang,X. Shawn Liu,X. Shawn Liu,Véronique Montplaisir,Mario Tiberi,Mario Tiberi,Jay M. Baltz,Jay M. Baltz,X. Johné Liu +11 more
TL;DR: It is demonstrated that ritanserin (RIT), a potent antagonist of serotonin receptors 5‐HT2R and 5-HT7R, released G2 arrest in denuded frog oocytes, as well as in follicle‐enclosed mouse oocytes.
Book ChapterDOI
Invention and Early History of Morpholinos: From Pipe Dream to Practical Products.
TL;DR: With improved delivery techniques, such as those in development at Gene Tools, therapeutic Morpholinos for many difficult-to-treat diseases will be possible and initial applications are expected to be custom cocktails of delivery-enabled Morpholines for treating cancers.
Journal ArticleDOI
A Matter of the Heart: The African Clawed Frog Xenopus as a Model for Studying Vertebrate Cardiogenesis and Congenital Heart Defects
Annemarie Hempel,Michael Kühl +1 more
TL;DR: The similarities between Xenopus and mammalian cardiogenesis are outlined, and an overview of well-studied cardiac genes in Xenopus are provided, which have been associated with congenital heart conditions.
Patent
Rna modulating oligonucleotides with improved characteristics for the treatment of duchenne and becker muscular dystrophy
TL;DR: The current invention provides animproved oligonucleotide and its use for treating, ameliorating, preventing and/or delaying DMD or BMD as discussed by the authors...
Book ChapterDOI
Morpholinos and PNAs Compared
TL;DR: This chapter will compare and contrast the properties and applications of two leading antisense molecules, Peptide Nucleic Acids (PNAs) and Morpholinos3,4 and where appropriate, the compelling advantages which these two advanced ‘blocker’ types provide relative to ‘modifier’types of antisense molecule.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.