Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
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TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
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Advancement of Nucleic Acid Biosensors Based on Morpholino
TL;DR: In this review, the characteristics of morpholino are briefly introduced, followed by highlights of nucleic acid biosensors based on Morpholino, including fabrication, analytical characteristics and biological applications.
Translational research of the quaking gene : Focusing on the conjunction between development and disease
TL;DR: Quaking (QKI) is an RNA binding protein involved in the post-transcriptional regulation of gene expression that has been identified as the cause of hypomyelination in a mouse mutant.
Journal ArticleDOI
ATP7B antisense oligodeoxynucleotides increase the cisplatin sensitivity of human ovarian cancer cell line SKOV3ipl.
TL;DR: ASODN transfection can inhibit the expression of ATP7B and increase the cisplatin sensitivity in SKOV3ip1 cells, while no significant changes were detected for groups treated with SODN/LF and LF only.
Journal ArticleDOI
Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA.
TL;DR: Morpholino oligonucleotides that have the same base sequences as any particular EGS will not direct cleavage by RNase P of the target RNA at the expected site in three mRNAs, but a PMO is just as efficient as a PPMO on a mRNA that is mutated so that the canonical W-C site has been altered.
Journal ArticleDOI
A newly identified Rab-GDI paralogue has a role in neural development in amphibia.
Liliya Nazlamova,Anna Noble,Frank Schubert,John McGeehan,Fiona A. Myers,Matthew Guille,Garry Scarlett +6 more
TL;DR: The analysis suggests the evolutionary origins of GDI3 and that it is functionally distinct from GDI1, and suggests that the key difference between GDI 1 and GDI 3 lies in their lipid binding pockets.
References
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Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
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The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
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Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
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Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.