Journal ArticleDOI
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
Reads0
Chats0
TLDR
In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1999-12-10. It has received 689 citations till now. The article focuses on the topics: RNase P & RNase H.read more
Citations
More filters
Journal ArticleDOI
Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish.
Jason Kuan Han Lai,Kristina K. Gagalova,Carsten Kuenne,Mohamed A. El-Brolosy,Didier Y.R. Stainier +4 more
TL;DR: Data show that morpholinos can induce the expression of ISGs in zebrafish embryos and further the understanding of morpholino effects.
Journal ArticleDOI
Sugar–thioacetamide backbone in oligodeoxyribonucleosides for specific recognition of nucleic acids
TL;DR: The amide linkage being shorter than the natural phosphate linkage, an additional atom is introduced into oligodeoxyribonucleosides with sugar-thioacetamide backbone that show very good RNA recognition properties.
Patent
Chemo- and radiation-sensitization of cancer by antisense trpm-2 oligodeoxynucleotides
TL;DR: In this paper, an improved method for treatment of renal cell cancer, which is generally resistant to treatment with known chemotherapy agents, was proposed by using an antisense oligodeoxynucleotides (ODN) targeted against the testosterone-repressed prostate message-2 (TRPM-2) gene.
Journal ArticleDOI
Analysis of circulating non-coding RNAs in a non-invasive and cost-effective manner.
Yu-Min Wang,Yu-Min Wang,Michael Patrick Trinh,Yongzan Zheng,Kaizhu Guo,Luis A. Jimenez,Wenwan Zhong +6 more
TL;DR: This review summarizes the recent developments in this area, highlighting the works devoted to cancer diagnosis and prognosis, and three main directions are focused: Extraction and purification of ncRNAs from body fluids; Quantification of the purified circulating nc RNAs; and Microfluidic platforms for integration of both steps to enable point-of-care diagnostics.
Journal ArticleDOI
Oligonucleotide-Based Therapy for FTD/ALS Caused by the C9orf72 Repeat Expansion: A Perspective.
Stephanie A. Fernandes,Andrew G. L. Douglas,Miguel A. Varela,Matthew J.A. Wood,Yoshitsugu Aoki +4 more
TL;DR: Antisense oligonucleotides are modified nucleic acids that are able to silence targeted mRNAs or perform splice modulation, and the fact that they have proved efficient in repeat expansion diseases including myotonic dystrophy type 1 makes them ideal candidates for c9FTD/ALS therapy.
References
More filters
Journal ArticleDOI
Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
Journal ArticleDOI
Intercellular trafficking and protein delivery by a herpesvirus structural protein.
Gillian Elliott,Peter O'Hare +1 more
TL;DR: It is shown that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin.
Journal ArticleDOI
Evaluation of 2'-modified oligonucleotides containing 2'-deoxy gaps as antisense inhibitors of gene expression
Brett P. Monia,Elena A. Lesnik,Carolyn Gonzalez,Walt F. Lima,Daniel Peter Claude Mcgee,Charles J. Guinosso,Andrew Mamoro Kawasaki,Phillip Dan Cook,Susan M. Freier +8 more
TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.