Myogenic factors that regulate expression of muscle-specific microRNAs
TLDR
This work suggests that induction of these microRNAs is important in regulating the expression of muscle-specific proteins, and demonstrates that the myogenic factors Myogenin and MyoD bind to regions upstream of thesemicroRNAs and are likely to regulate their expression.Abstract:
Since their discovery as key regulators of early animal development, microRNAs now are recognized as widespread regulators of gene expression. Despite their abundance, little is known regarding the regulation of microRNA biogenesis. We show that three highly conserved muscle-specific microRNAs, miR-1, miR-133 and miR-206, are robustly induced during the myoblast-myotube transition, both in primary human myoblasts and in the mouse mesenchymal C2C12 stem cell line. These microRNAs were not induced during osteogenic conversion of C2C12 cells. Moreover, both loci encoding miR-1, miR-1-1, and miR-1-2, and two of the three encoding miR-133, miR-133a-1 and miR-133a-2, are strongly induced during myogenesis. Some of the induced microRNAs are in intergenic regions, whereas two are transcribed in the opposite direction to the nonmuscle-specific gene in which they are embedded. By using CHIP analysis, we demonstrate that the myogenic factors Myogenin and MyoD bind to regions upstream of these microRNAs and, therefore, are likely to regulate their expression. Because miR-1 and miR-206 are predicted to repress similar mRNA targets, our work suggests that induction of these microRNAs is important in regulating the expression of muscle-specific proteins.read more
Citations
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Journal ArticleDOI
Biogenesis of small RNAs in animals.
TL;DR: This Review summarizes the current knowledge of how these intriguing molecules are generated in animal cells.
Journal ArticleDOI
A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
Marcella Cesana,Davide Cacchiarelli,Ivano Legnini,Tiziana Santini,Olga Sthandier,Mauro Chinappi,Anna Tramontano,Anna Tramontano,Irene Bozzoni +8 more
TL;DR: It is demonstrated that linc-MD1 exerts the same control over differentiation timing in human myoblasts, and that its levels are strongly reduced in Duchenne muscle cells, indicating that the ceRNA network plays an important role in muscle differentiation.
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Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis
Tsung Cheng Chang,Erik A. Wentzel,Oliver A. Kent,Kalyani Ramachandran,Michael Mullendore,Kwang Hyuck Lee,Georg Feldmann,Munekazu Yamakuchi,Marcella Ferlito,Charles J. Lowenstein,Dan E. Arking,Michael A. Beer,Anirban Maitra,Joshua T. Mendell +13 more
TL;DR: It is demonstrated that microRNAs (miRNAs) are important components of the p53 transcriptional network and miR-34a-responsive genes are highly enriched for those that regulate cell-cycle progression, apoptosis, DNA repair, and angiogenesis.
Journal ArticleDOI
MicroRNA-133 controls cardiac hypertrophy
Alessandra Carè,Daniele Catalucci,Federica Felicetti,Désirée Bonci,Antonio Addario,Paolo Gallo,Marie Louise Bang,Patrizia Segnalini,Yusu Gu,Nancy D. Dalton,Leonardo Elia,Michael V.G. Latronico,Morten A. Høydal,Camillo Autore,Matteo Antonio Russo,Gerald W. Dorn,Øyvind Ellingsen,Pilar Ruiz-Lozano,Kirk L. Peterson,Carlo M. Croce,Cesare Peschle,Gianluigi Condorelli +21 more
TL;DR: The data show thatmiR-133, and possibly miR-1, are key regulators of cardiac hypertrophy, suggesting their therapeutic application in heart disease.
Journal ArticleDOI
The evolution of gene regulation by transcription factors and microRNAs
Kevin Chen,Nikolaus Rajewsky +1 more
TL;DR: This work proposes a simple model that describes the transcriptional regulation of new microRNAs, a large class of small, non-coding RNAs in plants and animals, focusing on the evolution of the individual regulators and their binding sites.
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