Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model.
Ana Paula D. N. de Barros,Christina Maeda Takiya,Luciana Ribeiro Garzoni,Mona Lisa Leal-Ferreira,Hélio S. Dutra,Luciana B. Chiarini,Maria de Nazareth Leal de Meirelles,Radovan Borojevic,Maria Isabel D. Rossi +8 more
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TLDR
Undifferentiated and one-week osteo-induced BMSC self-assembled in a 3D spheroid and formed a microenvironment that is informative for hematopoietic progenitor cells, allowing their lodgment and controlling their proliferation.Abstract:
Background Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell population in HSC fate have not been established. In vivo models are complex and involve subtle species-specific differences, while bidimensional cultures do not reflect the 3D tissue organization. The aim of this study was to investigate in vitro the role of human bone marrow-derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs. Methodology/principal findings A complex mixed multicellular spheroid in vitro model was developed with human BMSC, undifferentiated or induced for one week into osteoblasts. A clear limit between the two stromal cells was established, and deposition of extracellular matrix proteins fibronectin, collagens I and IV, laminin, and osteopontin was similar to the observed in vivo. Noninduced BMSC cultured as spheroid expressed higher levels of mRNA for the chemokine CXCL12, and the growth factors Wnt5a and Kit ligand. Cord blood and bone marrow CD34(+) cells moved in and out the spheroids, and some lodged at the interface of the two stromal cells. Myeloid colony-forming cells were maintained after seven days of coculture with mixed spheroids, and the frequency of cycling CD34(+) cells was decreased. Conclusions/significance Undifferentiated and one-week osteo-induced BMSC self-assembled in a 3D spheroid and formed a microenvironment that is informative for hematopoietic progenitor cells, allowing their lodgment and controlling their proliferation.read more
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A Novel Model of Dormancy for Bone Metastatic Breast Cancer Cells
Rebecca Marlow,Gabriella Honeth,Sara Lombardi,Massimiliano Cariati,Sonya Hessey,Aikaterini Pipili,V. Mariotti,Bharath Buchupalli,Katie Foster,Dominique Bonnet,Agamemnon E. Grigoriadis,Pranela Rameshwar,Anand D. Purushotham,Andrew Tutt,Gabriela Dontu +14 more
TL;DR: Novel experimental systems are established that model the bone microenvironment of the breast cancer metastatic niche based on 3D cocultures of breast cancer cells with cell types predominant in bone marrow that will be instrumental for metastasis studies, particularly the study of cellular dormancy.
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SLAM Family Receptors Distinguish Hematopoietic Stem and Progenitor Cells and Reveal Endothelial Niches for Stem Cells
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