Porcine methionine sulfoxide reductase B3: molecular cloning, tissue-specific expression profiles, and polymorphisms associated with ear size in Sus scrofa
Yuebo Zhang,Jing Liang,Longchao Zhang,Ligang Wang,Xin Liu,Hua Yan,Kebin Zhao,Huibi Shi,Tian Zhang,Na Li,Lei Pu,Lixian Wang +11 more
Reads0
Chats0
TLDR
The porcine MSRB3 owned a 3,765-bp full-length cDNA sequence and was detected to express in ear tissue and two SNPs of this gene were shown to be significantly associated with ear size in a Large White’× Minzhu intercross population instead of Beijing Black pig population.Abstract:
In Sus scrofa, methionine sulfoxide reductase B3 (MSRB3) is a crucial candidate gene for ear size, and an important conformational trait of pig breeds. However, challenges in MSRB3 cDNA amplification have prevented further identification of MSRB3 allelic variants influencing pig ear size. We cloned a full-length cDNA sequence of porcine MSRB3 by rapid-amplification of cDNA ends. The 3,765-bp gene contained a 5’-untranslated region (UTR) (190 bp), a coding region (552 bp), and a 3’-UTR (3,016 bp) and shared 84 %, 84 %, 87 %, 86 %, and 70 % sequence identities with human, orangutan, mouse, chicken, and zebrafish, respectively. The gene encoded a 183-amino acid protein, which shared 88 %, 91 %, 89 %, 86 %, and 67 % identities with human, orangutan, mouse, chicken, and zebrafish, respectively. Tissue expression analysis using qRT-PCR revealed that MSRB3 was expressed in the heart, liver, lung, kidney, spleen, ear, muscle, fat, lymph, skeletal, and hypothalamic tissues. Three single nucleotide polymorphisms (SNPs) were identified in MSRB3: c.-735C > T in the 5’ flanking region, c.2571 T > C in the 3’-UTR, and a synonymous mutation of c.484 T > C in the coding region. The SNPs c.-735C > T and c.2571 T > C were significantly associated with ear size in a Large White × Minzhu F2 population other than in Beijing Black pigs. Subsequently, at SNP c.-735C > T, the mRNA of MSRB3 was significantly higher expressed in ears of individuals with the TT genotype (Minzhu) than those with CC (Large White). The porcine MSRB3 owned a 3,765-bp full-length cDNA sequence and was detected to express in ear tissue. Two SNPs of this gene were shown to be significantly associated with ear size in a Large White × Minzhu intercross population instead of Beijing Black pig population. What’s more, the individuals with higher mRNA expression of MSRB3 have larger ear sizes. These results provide useful information for further functional analyses of MSRB3 influencing ear size in pigs.read more
Citations
More filters
Journal ArticleDOI
Revealing the selection history of adaptive loci using genome-wide scans for selection: an example from domestic sheep
Christina Marie Rochus,Christina Marie Rochus,Christina Marie Rochus,Flavie Tortereau,Florence Plisson-Petit,Gwendal Restoux,Carole Moreno-Romieux,Gwenola Tosser-Klopp,Bertrand Servin +8 more
TL;DR: This study illustrates how dense genetic data in multiple populations allows the deciphering of evolutionary history of populations and of their adaptive mutations.
Journal ArticleDOI
Genomic signatures of high-altitude adaptation in Ethiopian sheep populations
TL;DR: Functional analyses reveal that the candidate genes involved in biological processes and pathways relevant to adaptation under extreme altitudes, including respiratory system development and smoothened signaling pathway could aid in-depth understanding and exploitation of the underlying genetic mechanisms for sheep and other livestock species adaptation to high-altitude environments.
Journal ArticleDOI
Sulphur amino acids, muscle redox status and meat quality: More than building blocks - Invited review.
TL;DR: The comprehension of these chemistry fundamentals enables a rationale manipulation of SAA via animal nutrition and/or food formulation to guarantee meat quality and safety and furthermore, develop novel, safer and more nutritious muscle foods, with increased oxidative stability.
Common variants at 12q14 and 12q24 are associated with hippocampal volume
Joshua C. Bis,Charles DeCarli,Albert V. Smith,Fedde van der Lijn,Fabrice Crivello,Fabrice Crivello,Myriam Fornage,Stephanie Debette,Stephanie Debette,Joshua M. Shulman,Joshua M. Shulman,Helena Schmidt,Velandai Srikanth,Velandai Srikanth,Maaike Schuur,Lei Yu,Seung Hoan Choi,Sigurdur Sigurdsson,Benjamin F.J. Verhaaren,Anita L. DeStefano,Jean-Charles Lambert,Clifford R. Jack,Maksim Struchalin,Jim Stankovich,Carla A. Ibrahim-Verbaas,Debra A. Fleischman,Alex P. Zijdenbos,Tom den Heijer,Bernard Mazoyer,Bernard Mazoyer,Laura H. Coker,Christian Enzinger,Patrick Danoy,Najaf Amin,Konstantinos Arfanakis,Konstantinos Arfanakis,Mark A. van Buchem,Renée F A G de Bruijn,Alexa S. Beiser,Carole Dufouil,Juebin Huang,Margherita Cavalieri,Russell Thomson,Wiro J. Niessen,Wiro J. Niessen,Lori B. Chibnik,Lori B. Chibnik,Gauti Kjartan Gislason,Albert Hofman,Aleksandra Pikula,Philippe Amouyel,Kevin B. Freeman,Thanh G. Phan,Ben A. Oostra,Jason L. Stein,Sarah E. Medland,Sarah E. Medland,Alejandro Arias Vasquez,Derrek P. Hibar,Margaret J. Wright,Barbara Franke,Nicholas G. Martin,Paul M. Thompson,Mike A. Nalls,André G. Uitterlinden,Rhoda Au,Alexis Elbaz,Richard Beare,Richard Beare,John C. van Swieten,Oscar L. Lopez,Tamara B. Harris,Vincent Chouraki,Monique M.B. Breteler,Monique M.B. Breteler,Monique M.B. Breteler,Philip L. De Jager,Philip L. De Jager,James T. Becker,Meike W. Vernooij,David S. Knopman,Franz Fazekas,Philip A. Wolf,Aad van der Lugt,Vilmundur Gudnason,William T. Longstreth,Matthew A. Brown,David A. Bennett,Cornelia M. van Duijn,Cornelia M. van Duijn,Thomas H. Mosley,Reinhold Schmidt,Christophe Tzourio,Christophe Tzourio,Lenore J. Launer,M. Arfan Ikram,Sudha Seshadri +96 more
TL;DR: These associations implicate genes related to apoptosis (HRK), development (WIF1), oxidative stress (MSR3B), ubiquitination (FBXW8) and neuronal migration (ASTN2), as well as enzymes targeted by new diabetes medications (DPP4), indicating new genetic influences on hippocampal size and possibly the risk of cognitive decline and dementia.
Journal ArticleDOI
Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
Congying Chen,Chenlong Liu,Xinwei Xiong,Shaoming Fang,Hui Yang,Zhiyan Zhang,Jun Ren,Yuanmei Guo,Lusheng Huang +8 more
TL;DR: The presence of the 38.7-kb CNV in the genome of some pig breeds affects ear size by altering the expression of miR-584-5p, which consequently hinders theexpression of one of its target genes (e.g. MSRB3).
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Book ChapterDOI
DNASTAR's Lasergene sequence analysis software.
TL;DR: The Lasergene software suite provides the functions and customization tools needed so that users can perform analyses the software writers never imagined.
Journal ArticleDOI
Alternative pre-mRNA splicing: the logic of combinatorial control.
TL;DR: Alternative splicing of mRNA precursors is a versatile mechanism of gene expression regulation that accounts for a considerable proportion of proteomic complexity in higher eukaryotes.
Related Papers (5)
Identification of Genomic Regions Associated with Phenotypic Variation between Dog Breeds using Selection Mapping
Amaury Vaysse,Abhirami Ratnakumar,Thomas Derrien,Erik Axelsson,Gerli Pielberg,Snaevar Sigurdsson,Tove Fall,Eija H. Seppälä,Mark Hansen,Cindy Lawley,Elinor K. Karlsson,Danika L. Bannasch,Carles Vilà,Hannes Lohi,Francis Galibert,Merete Fredholm,Jens Häggström,Åke Hedhammar,Catherine André,Kerstin Lindblad-Toh,Christophe Hitte,Matthew T. Webster +21 more
A Simple Genetic Architecture Underlies Morphological Variation in Dogs
Adam R. Boyko,Pascale Quignon,Lin Li,Jeffrey J. Schoenebeck,Jeremiah D. Degenhardt,Kirk E. Lohmueller,Keyan Zhao,Keyan Zhao,Abra Brisbin,Heidi G. Parker,Bridgett M. vonHoldt,Michele Cargill,Adam Auton,Andrew R. Reynolds,Abdel G. Elkahloun,Marta Castelhano,Dana S. Mosher,Nathan B. Sutter,Gary S. Johnson,John Novembre,Melissa J. Hubisz,Adam Siepel,Robert K. Wayne,Carlos Bustamante,Carlos Bustamante,Elaine A. Ostrander +25 more