PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells
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TLDR
Using in vivo RNA-protein cross-linking, it is shown that EZH2 directly binds the 5′ region of nascent RNAs transcribed from a subset of these promoters and that these binding events correlate with decreased H3K27me3.Abstract:
Polycomb repressive complex 2 (PRC2) acts as an epigenetic repressor by depositing repressive H3K27me3 marks, but how it is regulated and directed to specific genes remains unknown PRC2 is now found to bind at low levels to many gene promoters, including active ones devoid of H3K27me3, and the EZH2 catalytic subunit binds directly to nascent transcriptsread more
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A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element
Veronika A. Herzog,Adelheid Lempradl,Johanna Trupke,Helena Okulski,Christina Altmutter,Frank Ruge,Bernd Boidol,Stefan Kubicek,Gerald Schmauss,Karin Aumayr,Marius Ruf,Andrew Pospisilik,Andrew Dimond,Hasene Basak Senergin,Marcus L. Vargas,Jeffrey A. Simon,Leonie Ringrose +16 more
TL;DR: This work identifies a previously unreported and potentially widespread class of PRE/TREs that switch function by switching the direction of noncoding RNA transcription, allowing regulated inhibition of local PRC2 activity.
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MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing PCDH10.
Ying Qi,Hong Sain Ooi,Jun Wu,Jian Chen,Xiaoli Zhang,Sheng Tan,Qing Yu,Yuan-Yuan Li,Yani Kang,Hua Li,Zirui Xiong,Tao Zhu,Bingya Liu,Zhifeng Shao,Xiaodong Zhao +14 more
TL;DR: This work found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10, and promotes gastric cellular migration and invasion.
Journal ArticleDOI
Chromatin features and the epigenetic regulation of pluripotency states in ESCs
Wee-Wei Tee,Danny Reinberg +1 more
TL;DR: The current understanding of the role of chromatin as a plastic and integrative platform to direct gene expression changes in pluripotent stem cells, giving rise to distinct pluripotency states is reviewed.
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Systematic identification and characterization of long intergenic non-coding RNAs in fetal porcine skeletal muscle development
Weimin Zhao,Yulian Mu,Lei Ma,Chen Wang,Zhonglin Tang,Shulin Yang,Rong Zhou,Xiaoju Hu,Meng-Hua Li,Kui Li +9 more
TL;DR: It is found that the porcine lincRNAs were preferentially located near genes mediating transcriptional regulation rather than those with developmental functions, which indicates that the functions of these genes in the skeletal muscle developmental process are under investigation.
Journal ArticleDOI
Modulation of Chromatin by Noncoding RNA
TL;DR: Both long and short ncRNAs play key roles in organization of complex genomes of higher eukaryotes, and their coordinated actions appear to underlie some of the more dramatic examples of epigenetic regulation.
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