Showing papers on "Protein filament published in 2013"

Herschel view of the Taurus B211/3 filament and striations: evidence of filamentary growth?

Abstract: We present first results from the Herschel Gould Belt survey for the B211/L1495 region in the Taurus molecular cloud. Thanks to their high sensitivity and dynamic range, the Herschel images reveal the structure of the dense, star-forming filament B211 with unprecedented detail, along with the presence of striations perpendicular to the filament and generally oriented along the magnetic field direction as traced by optical polarization vectors. Based on the column density and dust temperature maps derived from the Herschel data, we find that the radial density profile of the B211 filament approaches power-law behavior, ρ ∝ r−2.0± 0.4, at large radii and that the temperature profile exhibits a marked drop at small radii. The observed density and temperature profiles of the B211 filament are in good agreement with a theoretical model of a cylindrical filament undergoing gravitational contraction with a polytropic equation of state: P ∝ ργ and T ∝ ργ−1, with γ = 0.97 ± 0.01 < 1 (i.e., not strictly isothermal). The morphology of the column density map, where some of the perpendicular striations are apparently connected to the B211 filament, further suggests that the material may be accreting along the striations onto the main filament. The typical velocities expected for the infalling material in this picture are ~0.5–1 km s-1, which are consistent with the existing kinematical constraints from previous CO observations.

Flagella and bacterial pathogenicity

Abstract: As locomotive organelles, flagella allow bacteria to move toward favorable environments A flagellum consists of three parts: the basal structure (rotary motor), the hook (universal joint), and the filament (helical propeller) For ages, flagella have been generally regarded as important virulence factors, mainly because of their motility property However, flagella are getting recognized to play multiple roles with more functions besides motility and chemotaxis Recent evidence has pinpointed that the bacterial flagella participate in many additional processes including adhesion, biofilm formation, virulence factor secretion, and modulation of the immune system of eukaryotic cells This mini-review summarizes data from recent studies that elucidated how flagella, as a virulence factor, contribute to bacterial pathogenicity

Filamentary accretion flows in the embedded serpens south protocluster

Abstract: One puzzle in understanding how stars form in clusters is the source of mass—is all of the mass in place before the first stars are born, or is there an extended period when the cluster accretes material which can continuously fuel the star formation process? We use a multi-line spectral survey of the southern filament associated with the Serpens South embedded cluster-forming region in order to determine if mass is accreting from the filament onto the cluster, and whether the accretion rate is significant. Our analysis suggests that material is flowing along the filament's long axis at a rate of ~30 M ☉ Myr–1 (inferred from the N2H+ velocity gradient along the filament), and radially contracting onto the filament at ~130 M ☉ Myr–1 (inferred from HNC self-absorption). These accretion rates are sufficient to supply mass to the central cluster at a similar rate to the current star formation rate in the cluster. Filamentary accretion flows may therefore be very important in the ongoing evolution of this cluster.

Variation in dust properties in a dense filament of the Taurus molecular complex (L1506)

Abstract: Aims. We observed the L1506 filament, which is located in the Taurus molecular complex, with the Herschel PACS and SPIRE instruments. Our aim is to prove the variation in grain properties along the entire length of the filament. In particular, we want to determine above which gas density this variation arises and what changes in the grain optical properties/size distribution are required.Methods. We use the 3D radiative transfer code CRT, coupled to the dust emission and extinction code DustEM, to model the emission and extinction of the dense filament. We test a range of optical properties and size distributions for the grains: dust of the diffuse interstellar medium (interstellar PAHs and amorphous carbons and silicates) and both compact and fluffy aggregates.Results. We find that the grain opacity has to increase across the filament to fit simultaneously the near-IR extinction and Herschel emission profiles of L1506. We interpret this change to be a consequence of the coagulation of dust grains to form fluffy aggregates. Grains similar to those in the diffuse medium have to be present in the outer layers of the cloud, whereas aggregates must prevail above gas densities of a few 103 H/cm3. This corresponds to line-of-sights with visual extinction in the V band of the order of 2 to 3. The dust opacity at 250 μm is raised by a factor of 1.8 to 2.2, while the grain average size is increased by a factor of 5. These exact numbers depend naturally on the dust model chosen to fit the data. Our findings agree with the constraints given by the study of the gas molecular lines. Using a simple approach, we show that the aggregates may have time to form inside the filament within the cloud lifetime. Our model also characterises the density structure of the filament, showing that the filament width is not constant along L1506 but instead varies by a factor of the order of 4.Conclusions. We confirm the need for an increase in the far-IR dust opacity to explain the emission and extinction in L1506C, which we interpret as being due to dust growth. We also show that this opacity variation is valid along the entire length of the L1506 dense filament.

Structure of sunspot penumbral filaments: a remarkable uniformity of properties

Abstract: The sunspot penumbra comprises numerous thin, radially elongated filaments that are central for heat transport within the penumbra, but whose structure is still not clear. To investigate the fine-scale structure of these filaments, we perform a depth-dependent inversion of spectropolarimetric data of a sunspot very close to solar disk center obtained by Hinode (SOT/SP). We have used a recently developed spatially coupled 2D inversion scheme which allows us to analyze the fine structure of individual penumbral filaments up to the diffraction limit of the telescope. Filaments of different sizes in all parts of penumbra display very similar magnetic field strengths, inclinations and velocity patterns. The similarities allowed us to average all these filaments and to extract the physical properties common to all of them. This average filament shows upflows associated with an upward pointing field at its inner, umbral end and along its axis, downflows along the lateral edge and strong downflows in the outer end associated with a nearly vertical, strong and downward pointing field. The upflowing plasma is significantly hotter than the downflowing plasma. The hot, tear-shaped head of the averaged filament can be associated with a penumbral grain. The central part of the filament shows nearly horizontal fields with strengths of ~1kG. The field above the filament converges, whereas a diverging trend is seen in the deepest layers near the head of the filament. We put forward a unified observational picture of a sunspot penumbral filament. It is consistent with such a filament being a magneto-convective cell, in line with recent MHD simulations. The uniformity of its properties over the penumbra sets constraints on penumbral models and simulations. The complex and inhomogeneous structure of the filament provides a natural explanation for a number of long-running controversies in the literature.

Stability of viscous long liquid filaments

Abstract: We study the collapse of an axisymmetric liquid filament both analytically and by means of a numerical model. The liquid filament, also known as ligament, may either collapse stably into a single droplet or break up into multiple droplets. The dynamics of the filament are governed by the viscosity and the aspect ratio, and the initial perturbations of its surface. We find that the instability of long viscous filaments can be completely explained by the Rayleigh-Plateau instability, whereas a low viscous filament can also break up due to end pinching. We analytically derive the transition between stable collapse and breakup in the Ohnesorge number versus aspect ratio phase space. Our result is confirmed by numerical simulations based on the slender jet approximation and explains recent experimental findings by Castrejon-Pita et al.

Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation

Abstract: During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-color single-molecule imaging in vitro we revealed how the dynamic associations of Arp2/3 complex with mother filament and WASP are temporally coordinated with initiation of daughter filament growth. We found that WASP proteins dissociated from filament-bound Arp2/3 complex prior to new filament growth. Further, mutations that accelerated release of WASP from filament-bound Arp2/3 complex proportionally accelerated branch formation. These data suggest that while WASP promotes formation of pre-nucleation complexes, filament growth cannot occur until it is triggered by WASP release. This provides a mechanism by which membrane-bound WASP proteins can stimulate network growth without restraining it. DOI: http://dx.doi.org/10.7554/eLife.01008.001

Structure of sunspot penumbral filaments: a remarkable uniformity of properties

Abstract: Context. The sunspot penumbra comprises numerous thin, radially elongated filaments that are central for heat transport within the penumbra, but whose structure is still not clear. Aims. We aim to investigate the fine-scale structure of these penumbral filaments. Methods. We perform a depth-dependent inversion of spectropolarimetric data of a sunspot very close to solar disk center obtained by Solar Optical Telescope/Spectropolarimeter onboard the Hinode spacecraft. We have used a recently developed, spatially coupled 2D inversion scheme, which allows us to analyze the fine structure of individual penumbral filaments up to the diffraction limit of the telescope. Results. Filaments of different sizes in all parts of the penumbra display very similar magnetic field strengths, inclinations, and velocity patterns. The temperature structure is also similar, although the filaments in the inner penumbra have cooler tails than those in the outer penumbra. The similarities allowed us to average all these filaments and to subsequently extract the physical properties common to all of them. This average filament shows upflows associated with an upward-pointing field at its inner, umbral end (head) and along its axis, as well as downflows along the lateral edge and strong downflows in the outer end (tail) associated with a nearly vertical, strong, and downward-pointing field. The upflowing plasma is significantly, i.e., up to 800 K, hotter than the downflowing plasma. The hot, tear-shaped head of the averaged filament can be associated with a penumbral grain. The central part of the filament shows nearly horizontal fields with strengths in the range of 1 kG. The field above the filament converges, whereas a diverging trend is seen in the deepest layers near the head of the filament. The fluctuations in the physical parameters along and across the filament increase rapidly with depth. Conclusions. We put forward a unified observational picture of a sunspot penumbral filament. It is consistent with such a filament being a magneto-convective cell, in line with recent magnetohydrodynamic simulations. The uniformity of its properties over the penumbra sets constraints on penumbral models and simulations. The complex and inhomogeneous structure of the filament provides a natural explanation for a number of long-running controversies in the literature.

Shock-Enhanced C+ Emission and the Detection of H2O from Stephan's Quintet's Group-Wide Shock using Herschel

Abstract: We present the first Herschel spectroscopic detections of the [OI]63 and [CII]158 micron fine-structure transitions, and a single para-H2O line from the 35 x 15 kpc^2 shocked intergalactic filament in Stephan's Quintet. The filament is believed to have been formed when a high-speed intruder to the group collided with clumpy intergroup gas. Observations with the PACS spectrometer provide evidence for broad (> 1000 km s^-1) luminous [CII] line profiles, as well as fainter [OI]63micron emission. SPIRE FTS observations reveal water emission from the p-H2O (111-000) transition at several positions in the filament, but no other molecular lines. The H2O line is narrow, and may be associated with denser intermediate-velocity gas experiencing the strongest shock-heating. The [CII]/PAH{tot) and [CII]/FIR ratios are too large to be explained by normal photo-electric heating in PDRs. HII region excitation or X-ray/Cosmic Ray heating can also be ruled out. The observations lead to the conclusion that a large fraction the molecular gas is diffuse and warm. We propose that the [CII], [OI] and warm H2 line emission is powered by a turbulent cascade in which kinetic energy from the galaxy collision with the IGM is dissipated to small scales and low-velocities, via shocks and turbulent eddies. Low-velocity magnetic shocks can help explain both the [CII]/[OI] ratio, and the relatively high [CII]/H2 ratios observed. The discovery that [CII] emission can be enhanced, in large-scale turbulent regions in collisional environments has implications for the interpretation of [CII] emission in high-z galaxies.

Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging

Abstract: Actin filament nucleation by actin-related protein (Arp) 2/3 complex is a critical process in cell motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process. Triggered by the verprolin homology, central, and acidic (VCA) region of proteins in the Wiskott-Aldrich syndrome protein (WASp) family, Arp2/3 complex produces new (daughter) filaments as branches from the sides of preexisting (mother) filaments. We visualized individual fluorescently labeled Arp2/3 complexes dynamically interacting with and producing branches on growing actin filaments in vitro. Branch formation was strikingly inefficient, even in the presence of VCA: only ∼1% of filament-bound Arp2/3 complexes yielded a daughter filament. VCA acted at multiple steps, increasing both the association rate of Arp2/3 complexes with mother filament and the fraction of filament-bound complexes that nucleated a daughter. The results lead to a quantitative kinetic mechanism for branched actin assembly, revealing the steps that can be stimulated by additional cellular factors.

The length–tension curve in muscle depends on lattice spacing

Abstract: Classic interpretations of the striated muscle length–tension curve focus on how force varies with overlap of thin (actin) and thick (myosin) filaments. New models of sarcomere geometry and experiments with skinned synchronous insect flight muscle suggest that changes in the radial distance between the actin and myosin filaments, the filament lattice spacing, are responsible for between 20% and 50% of the change in force seen between sarcomere lengths of 1.4 and 3.4 µm. Thus, lattice spacing is a significant force regulator, increasing the slope of muscle's force–length dependence.

Origins of Rolling, Twisting, and Non-radial Propagation of Eruptive Solar Events

Abstract: We demonstrate that major asymmetries in erupting filaments and CMEs, namely major twists and non-radial motions are typically related to the larger-scale ambient environment around eruptive events. Our analysis of prominence eruptions observed by the STEREO, SDO, and SOHO spacecraft shows that prominence spines retain, during the initial phases, the thin ribbon-like topology they had prior to the eruption. This topology allows bending, rolling, and twisting during the early phase of the eruption, but not before. The combined ascent and initial bending of the filament ribbon is non-radial in the same general direction as for the enveloping CME. However, the non-radial motion of the filament is greater than that of the CME. In considering the global magnetic environment around CMEs, as approximated by the Potential Field Source Surface (PFSS) model, we find that the non-radial propagation of both erupting filaments and associated CMEs is correlated with the presence of nearby coronal holes, which deflect the erupting plasma and embedded fields. In addition, CME and filament motions, respectively, are guided towards weaker field regions, namely null points existing at different heights in the overlying configuration. Due to the presence of the coronal hole, the large-scale forces acting on the CME may be asymmetric. We find that the CME propagates usually non-radially in the direction of least resistance, which is always away from the coronal hole. We demonstrate these results using both low- and high-latitude examples.

Srs2 prevents Rad51 filament formation by repetitive motion on DNA.

Abstract: Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5'-3' direction. One Rad51 molecule binds to three nucleotides, and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2's DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation.

Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails

Abstract: The intracellular bacterial pathogen Listeria monocytogenes is capable of remodelling the actin cytoskeleton of its host cells such that “comet tails” are assembled powering its movement within cells and enabling cell-to-cell spread. We used cryo-electron tomography to visualize the 3D structure of the comet tails in situ at the level of individual filaments. We have performed a quantitative analysis of their supramolecular architecture revealing the existence of bundles of nearly parallel hexagonally packed filaments with spacings of 12–13 nm. Similar configurations were observed in stress fibers and filopodia, suggesting that nanoscopic bundles are a generic feature of actin filament assemblies involved in motility; presumably, they provide the necessary stiffness. We propose a mechanism for the initiation of comet tail assembly and two scenarios that occur either independently or in concert for the ensuing actin-based motility, both emphasizing the role of filament bundling.

Extrusion-based additive manufacturing system

Abstract: In a method of manufacturing an object, a filament is fed to an extrusion head. The filament has a semi-crystalline polymeric reinforcement portion and a polymeric matrix portion. The temperature of the filament is raised in the extrusion head above the melting point of the matrix portion but below the melting point of the reinforcement portion so that the matrix portion of the filament melts within the extrusion head, thereby forming a partially molten filament within the extrusion head. The reinforcement portion of the partially molten filament remains in a semi-crystalline state as it is extruded from the extrusion head. Relative movement is generated between the extrusion head and the substrate as the partially molten filament is extruded onto the substrate in order to form an extruded line on the substrate. The matrix portion of the extruded line solidifies after the extruded line has been formed on the substrate.

Bright Hot Impacts by Erupted Fragments Falling Back on the Sun: A Template for Stellar Accretion

Abstract: Impacts of falling fragments observed after the eruption of a filament in a solar flare on 7 June 2011 are similar to those inferred for accretion flows on young stellar objects. As imaged in the ultraviolet (UV)-extreme UV range by the Atmospheric Imaging Assembly onboard the Solar Dynamics Observatory, many impacts of dark, dense matter display uncommonly intense, compact brightenings. High-resolution hydrodynamic simulations show that such bright spots, with plasma temperatures increasing from ~10(4) to ~10(6) kelvin, occur when high-density plasma (>>10(10) particles per cubic centimeter) hits the solar surface at several hundred kilometers per second, producing high-energy emission as in stellar accretion. The high-energy emission comes from the original fragment material and is heavily absorbed by optically thick plasma, possibly explaining the lower mass accretion rates inferred from x-rays relative to UV-optical-near infrared observations of young stars.

Robocasting of alumina hollow filament lattice structures

Abstract: Robotic controlled deposition (robocasting) of an aqueous colloidal α-Al 2 O 3 gel for manufacturing of cellular ceramics with periodical lattice structure was investigated. The colloidal gel was loaded with 50 vol% α-Al 2 O 3 and exhibits shear-thinning behavior, a shear modulus of 288 kPa and a yield-stress of ∼700 Pa. Tubular filaments of circular and rectangular cross section having an outer diameter of 1.5 mm and a capillary diameter of 0.75 mm were deposited in an oil bath to fabricate lattice truss structures with free spanning filaments. After freeze drying the robocast grids were sintered in air at 1550 °C. X-ray μ-CT revealed continuity of the tubular filaments for long distances (∼650 mm). Critical conditions to avoid capillary collapse were discussed by considering bulging stress and pressure distribution within the hollow filament. At short filament length oil infiltration into the capillary driven by capillary suction supports the tubular filament whereas oil flow driven by movement of the tool nozzle causes pressure difference to increase linearly with increasing filament length.

Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin

Abstract: Lamellipodia are sheet-like protrusions formed during migration or phagocytosis and comprise a network of actin filaments. Filament formation in this network is initiated by nucleation/branching through the actin-related protein 2/3 (Arp2/3) complex downstream of its activator, suppressor of cAMP receptor/WASP-family verprolin homologous (Scar/WAVE), but the relative relevance of Arp2/3-mediated branching versus actin filament elongation is unknown. Here we use instantaneous interference with Arp2/3 complex function in live fibroblasts with established lamellipodia. This allows direct examination of both the fate of elongating filaments upon instantaneous suppression of Arp2/3 complex activity and the consequences of this treatment on the dynamics of other lamellipodial regulators. We show that Arp2/3 complex is an essential organizer of treadmilling actin filament arrays but has little effect on the net rate of actin filament turnover at the cell periphery. In addition, Arp2/3 complex serves as key upstream factor for the recruitment of modulators of lamellipodia formation such as capping protein or cofilin. Arp2/3 complex is thus decisive for filament organization and geometry within the network not only by generating branches and novel filament ends, but also by directing capping or severing activities to the lamellipodium. Arp2/3 complex is also crucial to lamellipodia-based migration of keratocytes.

Ion-neutral friction and accretion-driven turbulence in self-gravitating filaments

Abstract: Recent Herschel observations have confirmed that filaments are ubiquitous in molecular clouds and suggest, that irrespective of the column density, there is a characteristic width of about 0.1 pc whose physical origin remains unclear. We develop an analytical model that can be applied to self-gravitating accreting filaments. It is based on the one hand on the virial equilibrium of the central part of the filament and on the other hand on the energy balance between the turbulence driven by accretion onto the filament and dissipation. We consider two dissipation mechanisms, the turbulent cascade and the ion-neutral friction. Our model predicts that the width of the inner part of the filament is almost independent of the column density and leads to values comparable to what is inferred observationally if dissipation is due to ion-neutral friction. On the contrary, turbulent dissipation leads to a structure that is bigger and depends significantly on the column density. Our model provides a reasonable physical explanation which could explain the observed filament width when they are self-gravitating. It predicts the correct order or magnitude though hampered by some uncertainties.

Anti-parallel euv flows observed along active region filament threads with hi-c

Abstract: Plasma flows within prominences/filaments have been observed for many years and hold valuable clues concerning the mass and energy balance within these structures. Previous observations of these flows primarily come from Hα and cool extreme-ultraviolet (EUV) lines (e.g., 304 A) where estimates of the size of the prominence threads has been limited by the resolution of the available instrumentation. Evidence of "counter-steaming" flows has previously been inferred from these cool plasma observations, but now, for the first time, these flows have been directly imaged along fundamental filament threads within the million degree corona (at 193 A). In this work, we present observations of an AR filament observed with the High-resolution Coronal Imager (Hi-C) that exhibits anti-parallel flows along adjacent filament threads. Complementary data from the Solar Dynamics Observatory (SDO)/Atmospheric Imaging Assembly (AIA) and Helioseismic and Magnetic Imager are presented. The ultra-high spatial and temporal resolution of Hi-C allow the anti-parallel flow velocities to be measured (70-80 km s–1) and gives an indication of the resolvable thickness of the individual strands (0.''8 ± 0.''1). The temperature of the plasma flows was estimated to be log T (K) = 5.45 ± 0.10 using Emission Measure loci analysis. We find that SDO/AIA cannot clearly observe these anti-parallel flows or measure their velocity or thread width due to its larger pixel size. We suggest that anti-parallel/counter-streaming flows are likely commonplace within all filaments and are currently not observed in EUV due to current instrument spatial resolution.

The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation

Abstract: Septins are guanine nucleotide-binding proteins that polymerize into filamentous and higher-order structures. Cdc42 and its effector Gic1 are involved in septin recruitment, ring formation and dissociation. The regulatory mechanisms behind these processes are not well understood. Here, we have used electron microscopy and cryo electron tomography to elucidate the structural basis of the Gic1-septin and Gic1-Cdc42-septin interaction. We show that Gic1 acts as a scaffolding protein for septin filaments forming long and flexible filament cables. Cdc42 in its GTP-form binds to Gic1, which ultimately leads to the dissociation of Gic1 from the filament cables. Surprisingly, Cdc42-GDP is not inactive, but in the absence of Gic1 directly interacts with septin filaments resulting in their disassembly. We suggest that this unanticipated dual function of Cdc42 is crucial for the cell cycle. Based on our results we propose a novel regulatory mechanism for septin filament formation and dissociation. DOI: http://dx.doi.org/10.7554/eLife.01085.001.

A two-segment model for thin filament architecture in skeletal muscle

Abstract: Correct specification of myofilament length is essential for efficient skeletal muscle contraction. The length of thin actin filaments can be explained by a novel 'two-segment' model, wherein the thin filaments consist of two concatenated segments, which are of either constant or variable length. This is in contrast to the classic 'nebulin ruler' model, which postulates that thin filaments are uniform structures, the lengths of which are dictated by nebulin. The two-segment model implicates position-specific microregulation of actin dynamics as a general principle underlying actin filament length and stability.

The kelvin-helmholtz instability at coronal mass ejection boundaries in the solar corona: observations and 2.5d mhd simulations

Abstract: The Atmospheric Imaging Assembly on board the Solar Dynamics Observatory observed a coronal mass ejection with an embedded filament on 2011 February 24, revealing quasi-periodic vortex-like structures at the northern side of the filament boundary with a wavelength of approximately 14.4 Mm and a propagation speed of about 310 {+-} 20 km s{sup -1}. These structures could result from the Kelvin-Helmholtz instability occurring on the boundary. We perform 2.5D numerical simulations of the Kelvin-Helmholtz instability and compare the simulated characteristic properties of the instability with the observations, where we obtain qualitative as well as quantitative accordance. We study the absence of Kelvin-Helmholtz vortex-like structures on the southern side of the filament boundary and find that a magnetic field component parallel to the boundary with a strength of about 20% of the total magnetic field has stabilizing effects resulting in an asymmetric development of the instability.

Led light and filament thereof

Abstract: The invention discloses a LED lamp and filament thereof. The LED filament includes a substrate, a light emitting unit secured onto at least one side surface of the substrate, and a package adhesive layer surrounded on the periphery of the light emitting unit; the substrate is configured to be of an elongated bar construction; the light emitting unit comprises a plurality of blue light chips and red light chips regularly distributed on the substrate and sequentially connected to one another in series. For the LED filament of the invention, as the light emitting unit composed of blue and red light chips is disposed on the substrate, the LED filament has high color rendering and large light radiation angle.

Characterization of 3D filament dynamics in a MAST SOL flux tube geometry

Abstract: Non-linear simulations of filament propagation in a realistic MAST SOL flux tube geometry using the BOUT++ fluid modelling framework show an isolation of the dynamics of the filament in the divertor region from the midplane region due to three features of the magnetic geometry; the variation of magnetic curvature along the field line, the expansion of the flux tube and strong magnetic shear. Of the three effects, the latter two lead to a midplane ballooning feature of the filament, whilst the former leads to a ballooning around the X-points. In simulations containing all three effects the filament is observed to balloon at the midplane, suggesting that the role of curvature variation is sub-dominant to the flux expansion and magnetic shear. The magnitudes of these effects are all strongest near the X-point which leads to the formation of parallel density gradients. The filaments simulated, which represent filaments in MAST, are identified as resistive ballooning, meaning that their motion is inertially limited, not sheath limited. Parallel density gradients can drive the filament towards a Boltzmann response when the collisionalityof the plasma is low. The results here show that the formation of parallel density gradients is a natural and inevitable consequence of a realistic magnetic geometry and therefore the transition to the Boltzmann response is a consequence of the use of realistic magnetic geometry and does not require initializing specifically varying background profiles as in slab simulations. The filaments studied here are stable to the linear resistive drift-wave instability but are subject to the non-linear effects associated with the Boltzmann response, particularly Boltzmann spinning. The Boltzmann response causes the filament to spin on an axis. In later stages of its evolution a non-linear turbulent state develops where the vorticity evolves into a turbulent eddy field on the same length scale as the parallel current. The transition from interchange motion to the Boltzmann response occurs with increasing temperature through a decrease in collisionality. This is confirmed by measuring the correlation between density and potential perturbations within the filament, which is low in the antisymmetric state associated with the interchange mechanism, but high in the Boltzmann regime. In the Boltzmann regime net radial transport is drastically reduced whilst a small net toroidal transport is observed. This suggests that only a subset of filaments, those driven by the interchange mechanism at the separatrix, can propagate into the far SOL. Filaments in the Boltzmann regime will be confined to the near separatrix region and quickly disperse. It is plausible that filaments in both regimes can contribute to the SOL transport observed in experiment; the former by propagating the filament into the far SOL and the latter by dispersion of the density within the filament.

FMNL3 FH2-actin structure gives insight into formin-mediated actin nucleation and elongation

Abstract: Formins are actin-assembly factors that act in a variety of actin-based processes. The conserved formin homology 2 (FH2) domain promotes filament nucleation and influences elongation through interaction with the barbed end. FMNL3 is a formin that induces assembly of filopodia but whose FH2 domain is a poor nucleator. The 3.4-A structure of a mouse FMNL3 FH2 dimer in complex with tetramethylrhodamine-actin uncovers details of formin-regulated actin elongation. We observe distinct FH2 actin-binding regions; interactions in the knob and coiled-coil subdomains are necessary for actin binding, whereas those in the lasso-post interface are important for the stepping mechanism. Biochemical and cellular experiments test the importance of individual residues for function. This structure provides details for FH2-mediated filament elongation by processive capping and supports a model in which C-terminal non-FH2 residues of FMNL3 are required to stabilize the filament nucleus.