McGill University

About: McGill University is a education organization based out in Montreal, Quebec, Canada. It is known for research contribution in the topics: Population & Context (language use). The organization has 72688 authors who have published 162565 publications receiving 6966523 citations. The organization is also known as: Royal institution of advanced learning & University of McGill College.

Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes

Abstract: Plant growth promoting bacteria (PGPR) associations range in degree of bacterial proximity to the root and intimacy of association. In general, these can be separated into extracellular PGPR (ePGPR), existing in the rhizosphere, on the rhizoplane or in the spaces between cells of the root cortex, and intracellular PGPR (iPGPR), which exist inside root cells, generally in specialized nodular structures. The latter includes rhizobia and Frankia species, both of which fix nitrogen in symbiosis with higher plants. There has been considerable development in understanding signaling mechanisms of rhizobia (iPGPR) during the establishment of the rhizobia–legume symbiosis, and this may serve as a model of knowledge regarding cross-talk and plant growth promoting mechanisms. We provide a detailed review of this process, including plant-to-bacteria signal molecules, followed by bacterial perception and consequent production of bacteria-to-plant signals. A history of PGPR discovery is also provided, indicating progress in understanding each of the PGPR groups. Recent advances in understanding plant growth responses to microbial signals are reviewed, along with the research areas that require attention. Based on new understandings of signaling mechanisms in the iPGPR (rhizobia) and recent findings with ePGPR we are able to speculate regarding general patterns of signaling in the ePGPR.

4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway

Abstract: Growth factors and hormones activate protein translation by phosphorylation and inactivation of the translational repressors, the eIF4E-binding proteins (4E-BPs), through a wortmannin- and rapamycin-sensitive signaling pathway. The mechanism by which signals emanating from extracellular signals lead to phosphorylation of 4E-BPs is not well understood. Here we demonstrate that the activity of the serine/threonine kinase Akt/PKB is required in a signaling cascade that leads to phosphorylation and inactivation of 4E-BP1. PI 3-kinase elicits the phosphorylation of 4E-BP1 in a wortmannin- and rapamycin-sensitive manner, whereas activated Akt-mediated phosphorylation of 4E-BP1 is wortmannin resistant but rapamycin sensitive. A dominant negative mutant of Akt blocks insulin-mediated phosphorylation of 4E-BP1, indicating that Akt is required for the in vivo phosphorylation of 4E-BP1. Importantly, an activated Akt induces phosphorylation of 4E-BP1 on the same sites that are phosphorylated upon serum stimulation. Similar to what has been observed with serum and growth factors, phosphorylation of 4E-BP1 by Akt inhibits the interaction between 4E-BP1 and eIF-4E. Furthermore, phosphorylation of 4E-BP1 by Akt requires the activity of FRAP/mTOR. FRAP/mTOR may lie downstream of Akt in this signaling cascade. These results demonstrate that the PI 3-kinase-Akt signaling pathway, in concert with FRAP/mTOR, induces the phosphorylation of 4E-BP1.

Holography, thermodynamics, and fluctuations of charged AdS black holes

Abstract: The physical properties of Reissner-Nordstrom black holes in (n11)-dimensional anti-de Sitter spacetime are related, by a holographic map, to the physics of a class of n-dimensional field theories coupled to a background global current. Motivated by that fact, and the recent observations of the striking similarity between the thermodynamic phase structure of these black holes ~in the canonical ensemble! and that of the van der Waals-Maxwell liquid-gas system, we explore the physics in more detail. We study fluctuations and stability within the equilibrium thermodynamics, examining the specific heats and electrical permittivity of the holes, and consider the analogue of the Clayperon equation at the phase boundaries. Consequently, we refine the phase diagrams in the canonical and grand canonical ensembles. We study the interesting physics in the neighborhood of the critical point in the canonical ensemble. There is a second order phase transition found there, and that region is characterized by a Landau-Ginzburg model with A3 potential. The holographically dual field theories provide the description of the microscopic degrees of freedom which underlie all of the thermodynamics, as can be seen by examining the form of the microscopic fluctuations. @S0556-2821~99!06820-4#